Test apparatus

• US 10,900,011 B2
• Filed: 09/19/2014
• Issued: 01/26/2021
• Est. Priority Date: 03/19/2014
• Status: Active Grant

• Current Assignee: Hitachi High-Tech Company Limited (Hitachi, Ltd.)
• Original Assignee: Hitachi High-Tech Company Limited (Hitachi, Ltd.)
• Inventors: Uematsu, Chihiro, Maeshima, Muneo, Masuya, Akira
• Primary Examiner(s): Hurst, Jonathan M
• Application Number:
  US15/125,980
• Publication Number:
  US 20170096631A1
• Time in Patent Office: 2,321 Days
• CPC Class Codes: C12M 23/12 Well or multiwell plates C12M25/04 takes precedence
• CPC Class Codes: C12M 41/12 of temperature controlling the temperature of chemical or physical processes B01J19/0013, heating or cooling apparatus for laboratory use B01L7/00
• CPC Class Codes: C12M 41/36 of biomass, e.g. colony counters or by turbidity measurements electrooptical investigation of individual particles G01N15/14, flow cytometers G01N15/1404
• CPC Class Codes: C12M 41/46 of cellular or enzymatic activity or functionality, e.g. cell viability
• CPC Class Codes: C12Q 1/04 Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor C12Q1/6897 takes precedence

Abstract

Provided is a test apparatus in which a test for bacterial identification or antimicrobial susceptibility can be promptly determined. A division state of bacteria is monitored by performing microscopic observation of shapes and the number of the bacteria in each of wells in a culture plate for bacterial identification culture or an antimicrobial susceptibility test, and it is determined whether or not the bacteria grow in a stage shifted from an induction phase to a logarithmic phase, with reference to an image obtained through microscopic observation. In addition, determination performed based on turbidity in the related art may be combined with determination performed based on microscopic observation in which change and the like in the shapes of the bacteria are monitored. Accordingly, it is possible to realize a highly accurate test result.

Claims

1. A test apparatus which is configured to perform an identification test or an antimicrobial susceptibility test of bacteria or fungi, the test apparatus comprising:

a temperature control unit that is configured to control a set temperature of a culture plate having a plurality of wells and retaining a culture liquid which contains the bacteria or the fungi in each well;

a microscopic observation optical system including a light source that outputs light and an objective lens having a magnification that allows observation of whether the bacteria or fungi is divided and through which the light passes after passing through the culture plate;

a transportation mechanism that is configured to transport the culture plate between the temperature control unit and the microscopic observation optical system, and

a processor to process an image from the microscopic observation optical system,

wherein the microscopic observation optical system is configured to acquire a microscopic observation image of the bacteria or the fungi in the culture liquid included in each of the wells in the culture plate, and the processor is configured to perform image processing of edge detection and binarization on the image, to extract feature values including a number of bacteria or fungi present in the image, areas of the bacteria or fungi present in the image, an average area, a circularity, an aspect ratio, and a perimeter length of bacterium or fungus from the image after image processing, and to perform comparison at least one of a feature value to that of images in a database.

2. The test apparatus according to claim 1,

wherein the temperature control unit is configured to control the set temperature to culture the bacteria or the fungi in the culture liquid which is retained in each well of the culture plate, and

wherein the microscopic observation optical system is configured to allow microscopic observation of a bacterial suspension contained in the culture liquid which is retained in each well of the culture plate, and to enable the identification test or the antimicrobial susceptibility test of the bacteria or the fungi.

3. The test apparatus according to claim 1, further comprising a turbidity measurement optical system configured to perform measurement of turbidity of the culture liquid that is retained in each well of the culture plate.

4. The test apparatus according to claim 3,

wherein the temperature control unit is configured to control the set temperature to culture the bacteria or the fungi in the culture liquid which is retained in each well of the culture plate,

wherein the microscopic observation optical system is configured to allow microscopic observation of the bacterial suspension contained in the culture liquid which is retained in each well of the culture plate, and to enable a first identification test or a first antimicrobial susceptibility test, and

wherein the turbidity measurement optical system is configured to allow measurement of turbidity of the bacterial suspension contained in the culture liquid which is retained in each well of the culture plate, and to enable a second identification test or a second antimicrobial susceptibility test.

5. The test apparatus according to claim 4,

wherein the microscopic observation optical system includes a light source and an imaging device that captures a transmission image of light emitted from the light source and having pass through the culture plate, and

at least some optical elements are used in common by the microscopic observation optical system and the turbidity measurement optical system.

6. The test apparatus according to claim 1, wherein:

the processor, in accordance with a program, is configured to perform matching of a division state of the bacteria or the fungi by comparing the image obtained by performing observation through the microscopic optical system with an image stored in a database in advance and performs determination of the identification test or the antimicrobial susceptibility test of the bacteria or the fungi.

7. The test apparatus according to claim 4,

wherein the processor is configured to extract a predetermined feature value of the bacteria or the fungi from the image obtained by performing observation through the microscopic optical system and to perform determination of the identification test or the antimicrobial susceptibility test of the bacteria or the fungi by comparing the extracted predetermined feature value with the predetermined feature value of the bacteria or the fungi extracted from the image in the database.

8. The test apparatus according to claim 1,

wherein the microscopic observation optical system includes a light source and an imaging device that captures a transmission image of light emitted from the light source and having passed through the culture plate, and acquires a microscopic image of the bacteria or the fungi in the culture liquid included in each of the wells in the culture plate, the microscopic observation image being a transmission image of each of the wells in the culture plate.