Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars

• US 10,907,183 B2
• Filed: 07/31/2019
• Issued: 02/02/2021
• Est. Priority Date: 04/30/2014
• Status: Active Grant

• Current Assignee: Versalis S.P.A, (Eni Petroleum Company Incorporated)
• Original Assignee: DSM IP Assets BV (DSM-Firmenich AG)
• Inventors: Noordam, Bertus, Berkhout, Michael Petrus Jozef
• Primary Examiner(s): Saidha, Tekchand
• Application Number:
  US16/528,191
• Publication Number:
  US 20200048669A1
• Time in Patent Office: 552 Days
• Field of Search: 435 99
• CPC Class Codes: C12P 19/02 Monosaccharides 2-ketogulonic acid C12P7/60
• CPC Class Codes: C12P 19/14 produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase , e.g. by cellulase, hemicellulase
• CPC Class Codes: C12P 2203/00 Fermentation products obtained from optionally pretreated or hydrolyzed cellulosic or lignocellulosic material as the carbon source ethanol C12P7/10
• CPC Class Codes: C13K 1/02 obtained by saccharification of cellulosic materials manufacture of fodder A23K10/32
• CPC Class Codes: C13K 13/00 Sugars not otherwise provided for in this class
• CPC Class Codes: C13K 13/002 Xylose
• CPC Class Codes: D21C 3/26 Multistage processes
• CPC Class Codes: Y02E 50/10 Biofuels, e.g. bio-diesel

Abstract

The invention relates to a process for the preparation of a fermentation product from lignocellulosic material, comprising the following steps:

• • a) optionally, pre-treatment of the lignocellulosic material,
  • b) optionally, washing of the optionally pretreated lignocellulosic material,
  • c) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises LPMO, and optionally purifying the hydrolysed lignocellulosic material,
  • d) fermentation of the hydrolysed lignocellulosic material to produce a fermentation product, and
  • e) optionally, recovery of a fermentation product,
    wherein oxygen is consumed in amounts corresponding to between 20 and 5000 mmol molecular oxygen per kg glucan present in the lignocellulosic material, the oxygen is added after the pretreatment and before and/or during the enzymatic hydrolysis of the lignocellulosic material, preferably in an amount corresponding to at least 30 mmol molecular oxygen per kg glucan present in the lignocellulosic material, more preferably in an amount corresponding to at least 40 mmol molecular oxygen per kg glucan present in the lignocellulosic material, and most preferably in an amount corresponding to at least 50 mmol molecular oxygen per kg glucan present in the lignocellulosic material is consumed.

Claims

1. A process for the preparation of a sugar product from lignocellulosic material comprising the following steps:

a) optionally, pretreatment of the lignocellulosic material,

b) optionally, washing of the optionally pretreated lignocellulosic material,

c) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises lytic polysaccharide monooxygenase (LPMO), and

d) optionally, recovery of a glucose-containing composition,

wherein oxygen is consumed in amounts corresponding to between 20 and 5000 mmol molecular oxygen per kg glucan present in the lignocellulosic material, the oxygen is added after the pretreatment and before and/or during the enzymatic hydrolysis of the lignocellulosic material.

5. The process of claim 1, wherein during the enzymatic hydrolysis step (c) oxygen is added to the lignocellulosic material.

6. The process of claim 1, wherein the oxygen is added in the form of bubbles.

7. The process of claim 1, wherein the reactor for the enzymatic hydrolysis has a volume of 1 m³ or more.

8. The process of claim 1, wherein the enzymatic hydrolysis time is 5 to 150 hours.

9. The process of claim 1, wherein the enzyme composition used retains activity for 30 hours or more.

10. The process of claim 1, wherein the hydrolysis is conducted at a temperature of 45°

C. or more, preferably 50°

C. or more and still more preferably at a temperature of 55°

C. or more.

11. The process of claim 1, wherein the enzyme composition is derived from a fungus, preferably a microorganism of the genus Rasamsonia or the enzyme composition comprises a fungal enzyme, preferably a Rasamsonia enzyme.

12. The process of claim 1, wherein the dry matter content in the hydrolysis step c is 10 wt % or more, preferably is 14 wt % or more and more preferably is 14 to 33 wt %.

13. The process of claim 1, in which wherein the enzymatic hydrolysis takes place in a batch, fed batch and/or continuous culture reactor.

14. The process of claim 1, wherein the enzyme composition is in the form of a whole fermentation broth of a fungus.

15. The process of claim 1, in which wherein the oxygen is introduced as an oxygen-containing gas, optionally air.

2. A process for the preparation of a fermentation product from lignocellulosic material comprising the following steps:

a) optionally, pretreatment of the lignocellulosic material,

b) optionally, washing of the optionally pretreated lignocellulosic material,

c) enzymatic hydrolysis of the optionally washed and/or optionally pretreated lignocellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises lytic polysaccharide monooxygenase (LPMO), and optionally purifying the hydrolysed lignocellulosic material,

d) fermentation of the hydrolysed lignocellulosic material to produce a fermentation product, and

e) optionally, recovery of a fermentation product,

wherein oxygen is consumed in amounts corresponding to between 20 and 5000 mmol molecular oxygen per kg glucan present in the lignocellulosic material, the oxygen is added after the pretreatment and before and/or during the enzymatic hydrolysis of the lignocellulosic material.

3. The process of claim 2, wherein the fermentation is conducted with a microorganism that is able to ferment at least one C5 sugar.

4. The process of claim 3, wherein the microorganism is of the species Saccharomyces cerevisiae, in which genetic modifications have been made.