Method and apparatus for in vivo surveillance of circulating biological components

• US 10,987,037 B2
• Filed: 09/23/2015
• Issued: 04/27/2021
• Est. Priority Date: 12/22/2003
• Status: Active Grant

• Current Assignee: John Wayne Cancer Institute
• Original Assignee: John Wayne Cancer Institute
• Inventors: Hoon, David, Taback, Bret, Shaolian, Samuel
• Primary Examiner(s): Lam, Ann Y
• Application Number:
  US14/863,332
• Publication Number:
  US 20160007896A1
• Time in Patent Office: 2,043 Days
• CPC Class Codes: A61B 2562/0285 Nanoscale sensors
• CPC Class Codes: A61B 5/14546 for measuring analytes not otherwise provided for, e.g. ions, cytochromes
• CPC Class Codes: A61B 5/14735 comprising an immobilised reagent
• CPC Class Codes: A61B 5/6851 Guide wires
• CPC Class Codes: A61B 5/6862 Stents
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: G01N 33/54366 Apparatus specially adapted for solid-phase testing

Abstract

The invention relates generally to in vivo collection of circulating molecules, tumor cells and other biological markers using a collecting probe. The probe is configured for placement within a living organism for an extended period of time to provide sufficient yield of biological marker for analysis.

Claims

1. A system for collecting markers associated with circulating biological components, comprising:

a probe comprising a guidewire comprising a binding surface and an atraumatic distal tip, wherein the binding surface comprises an outer nanostructured polymer zone and an inner non-porous zone, wherein the nanostructured polymer zone has affixed thereto at least one binding partner for binding a marker, and wherein the nanostructured polymer zone is configured to immobilize a marker to the binding surface upon binding of the marker to the binding partner, thereby increasing the surface area of the binding surface;

wherein at least a portion of the guidewire is configured to be placed within an anatomical structure of a living organism to collect the markers.

2. The system of claim 1, wherein the outer nanostructured polymer zone is a polymeric jacket.

3. The system of claim 1, wherein the probe further comprises at least one optically sensitive dye engaged to the binding surface.

4. The system of claim 1, wherein the probe further comprises a fibrin-deposition resistant component.

5. The system of claim 1, wherein the probe further comprises at least one anti-thrombotic agent engaged to the binding surface.

6. The system of claim 1, wherein the probe further comprises at least one antimicrobial agent engaged to the binding surface.

7. The system of claim 1, wherein the outer nanostructured polymer zone comprises a microporous surface having an average pore size of between about 5 microns and about 150 microns.

8. The system of claim 1, wherein the outer nanostructured polymer zone comprises a microporous surface having an average pore size of between about 10 microns and about 50 microns.

9. The system of claim 1, wherein the outer nanostructured polymer zone comprises a microporous surface having a porosity of between about 10% and about 80%.

10. The system of claim 7, wherein the microporous surface is directly bonded onto a solid surface of the probe.

11. The system of claim 1, wherein the guidewire comprises an average outer diameter of between about 0.5 mm and about 2 mm.

12. The system of claim 1, wherein the guidewire comprises a metallic material.

13. A system for collecting markers associated with circulating biological markers, comprising:

a probe comprising a guidewire comprising a binding surface and an atraumatic distal tip, wherein the binding surface comprises an outer microporous polymer zone and an inner non-porous zone, wherein the outer microporous polymer zone has affixed thereto at least one binding partner for binding a marker, and wherein the microporous polymer zone is configured to immobilize a marker to the binding surface upon binding of the marker to the binding partner, thereby increasing the surface area of the binding surface;

wherein at least a portion of the guidewire is configured to be placed within a blood vessel of a living organism to collect the markers.

14. The system of claim 13, wherein the at least one binding partner comprises an antibody.

15. The system of claim 13, wherein the at least one binding partner comprises a protein.

16. The system of claim 13, wherein the at least one binding partner comprises a nucleotide.

17. The system of claim 13, wherein the at least one binding partner comprises a nucleic acid.

18. A system for collecting markers associated with circulating biological markers, comprising:

a probe comprising a guidewire comprising a proximal end, binding surface, and an atraumatic distal tip, wherein the guidewire is configured to be implanted into a body lumen to bind at least one complementary target and ensure continuous contact with circulating body fluid within the body lumen, wherein the binding surface comprises an outer microporous polymer zone and an inner non-porous zone, wherein the outer microporous polymer zone has affixed thereto at least one binding partner for binding a marker, and wherein the microporous polymer zone is configured to immobilize a marker to the binding surface upon binding of the marker to the binding partner, thereby increasing the surface area of the binding surface;

wherein the guidewire has an external diameter of from about 0.025 inches to about 0.100 inches,

wherein the outer microporous polymer zone comprises an average pore size of between about 5 microns and about 150 microns.

19. The system of claim 18, further comprising a detection assembly.

20. The system of claim 18, wherein the outer microporous surface is directly bonded onto a solid surface of the probe.