Enzymatic production of D-tagatose
First Claim
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1. An enzymatic process for preparing tagatose from sucrose, the process comprising:
- (i) converting sucrose to glucose 1-phosphate (G1P) catalyzed by a sucrose phosphorylase (SP);
(ii) converting G1P to glucose 6-phosphate (G6P) catalyzed by a phosphoglucomutase (PGM);
(iii) converting G6P to fructose 6-phosphate (F6P) catalyzed by a phosphoglucoisomerase (PGI);
(iv) converting fructose 6-phosphate (F6P) to tagatose 6-phosphate (T6P) catalyzed by fructose 6-phosphate epimerase (F6PE); and
(v) converting the T6P produced to tagatose catalyzed by a tagatose 6-phospate phosphatase (T6PP).
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Abstract
The current disclosure provides a process for enzymatically converting a saccharide into tagatose. The invention also relates to a process for preparing tagatose where the process involves converting fructose 6-phosphate (F6P) to tagatose 6-phosphate (T6P), catalyzed by an epimerase, and converting the T6P to tagatose, catalyzed by a phosphatase.
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9 Claims
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1. An enzymatic process for preparing tagatose from sucrose, the process comprising:
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(i) converting sucrose to glucose 1-phosphate (G1P) catalyzed by a sucrose phosphorylase (SP); (ii) converting G1P to glucose 6-phosphate (G6P) catalyzed by a phosphoglucomutase (PGM); (iii) converting G6P to fructose 6-phosphate (F6P) catalyzed by a phosphoglucoisomerase (PGI); (iv) converting fructose 6-phosphate (F6P) to tagatose 6-phosphate (T6P) catalyzed by fructose 6-phosphate epimerase (F6PE); and (v) converting the T6P produced to tagatose catalyzed by a tagatose 6-phospate phosphatase (T6PP). - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9)
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Specification