Walnut oligopeptide powder, preparation method and application thereof

• US 11,072,637 B2
• Filed: 01/18/2017
• Issued: 07/27/2021
• Est. Priority Date: 01/22/2016
• Status: Active Grant

• Current Assignee: Sinphar Tian-Li (Hangzhou) Pharmacy Company Limited
• Original Assignee: Sinphar Tian-Li (Hangzhou) Pharmacy Company Limited
• Inventors: Wang, Zhaori, Liu, Mingchuan, Yang, Shengjie, Hong, Da, Yang, Jinping
• Primary Examiner(s): Katakam, Sudhakar
• Assistant Examiner(s): Miknis, Zachary J
• Application Number:
  US16/071,583
• Publication Number:
  US 20190276504A1
• Time in Patent Office: 1,651 Days
• Field of Search: None
• CPC Class Codes: A23J 1/148 by treatment involving enzymes or microorganisms enzymatic hydrolysis of proteins A23J3/34
• CPC Class Codes: A23J 3/346 of vegetable proteins
• CPC Class Codes: A23L 33/18 Peptides; Protein hydrolysates
• CPC Class Codes: A23L 33/185 Vegetable proteins
• CPC Class Codes: A23V 2002/00 Food compositions, function of food ingredients or processes for food or foodstuffs
• CPC Class Codes: A61K 38/00 Medicinal preparations containing peptides peptides containing beta-lactam rings A61K31/00; cyclic dipeptides not having in their molecule any other peptide link than those which form their ring, e.g. piperazine-2,5-diones, A61K31/00; ergot alkaloids of the cyclic peptide type A61K31/48; containing macromolecular compounds having statistically distributed amino acid units A61K31/74; medicinal preparations containing antigens or antibodies A61K39/00; medicinal preparations characterised by the non-active ingredients, e.g. peptides as drug carriers, A61K47/00
• CPC Class Codes: A61K 38/011 from plants
• CPC Class Codes: A61K 38/02 Peptides of undefined number of amino acids; Derivatives thereof
• CPC Class Codes: A61K 8/64 Proteins; Peptides; Derivatives or degradation products thereof
• CPC Class Codes: A61K 8/645 Proteins of vegetable origin; Derivatives or degradation products thereof
• CPC Class Codes: A61K 8/9789 Magnoliopsida [dicotyledons]
• CPC Class Codes: A61P 25/28 for treating neurodegenerative disorders of the central nervous system, e.g. nootropic agents, cognition enhancers, drugs for treating Alzheimer's disease or other forms of dementia
• CPC Class Codes: A61Q 19/00 Preparations for care of the skin
• CPC Class Codes: B01D 61/145 Ultrafiltration
• CPC Class Codes: B01D 61/147 Microfiltration
• CPC Class Codes: B01D 61/149 Multistep processes comprising different kinds of membrane processes selected from ultrafiltration or microfiltration
• CPC Class Codes: C07K 1/14 Extraction; Separation; Purification
• CPC Class Codes: C07K 1/34 by filtration, ultrafiltration or reverse osmosis
• CPC Class Codes: C07K 14/415 from plants
• CPC Class Codes: C07K 2/00 Peptides of undefined number of amino acids; Derivatives thereof
• CPC Class Codes: C12P 21/06 : produced by the hydrolysis of a peptide bond, e.g. hydrolysate products preparing foodstuffs by protein hydrolysis A23J3/00
• CPC Class Codes: Y02A 50/30 : Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The present invention provides a walnut oligopeptide powder, a preparation method and application thereof. The oligopeptide powder has a peptide content of 80 wt % or higher, in which 95% or more of the peptides have a molecular weight of less than 1,500 Da. The method includes extracting walnut proteins using a highly efficient countercurrent extraction process, and subjecting the extract to filtration, enzymolysis, purification with a microfiltration membrane and an ultrafiltration membrane, concentrating, and spray drying to obtain the oligopeptide powder. The oligopeptide powder has an anti-oxidant activity and provides a protective effect for a neuron, and thus can ameliorate or treat memory deterioration and relieve fatigue.

Claims

1. A composition, comprising:

a walnut oligopeptide powder, having a peptide content of more than 80 wt %, wherein more than 95% of the walnut oligopeptide has a molecular weight of less than 1500 Dalton, and a molecular weight distribution of the walnut peptide is as follows;