Caustic stable chromatography ligands

  • US 11,084,851 B2
  • Filed: 08/27/2018
  • Issued: 08/10/2021
  • Est. Priority Date: 12/24/2008
  • Status: Active Grant
First Claim
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1. An alkaline-stable chromatography ligand comprising two or more C domains of Staphylococcus protein A (SpA) attached to a chromatography resin at more than one site on the resin, wherein each C domain comprises the amino acid sequence set forth in SEQ ID NO:

  • 11 having a mutation to replace the glycine at position 29 with an amino acid other than alanine or tryptophan to reduce Fab binding, as shown in SEQ ID NO;

    44, wherein the ligand retains at least 95% of its binding capacity after 5 hours incubation in 0.5 M NaOH.

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