Open-field handheld fluorescence imaging systems and methods

  • US 11,140,305 B2
  • Filed: 05/10/2017
  • Issued: 10/05/2021
  • Est. Priority Date: 02/10/2017
  • Status: Active Grant
First Claim
Patent Images

1. A system for imaging a target, the system comprising:

  • one or more processors;

    memory; and

    one or more programs, wherein the one or more programs are stored in the memory and configured to be executed by the one or more processors, the one or more programs including instructions for, within a period;

    activating an excitation light source to generate an excitation pulse to illuminate the target;

    receiving an ambient light intensity signal from a non-imaging sensor during a portion of the period in which the excitation light source is not activated;

    exposing an image sensor for a fluorescent exposure time during the excitation pulse;

    receiving outputs from the image sensor;

    compensating for ambient light based on the ambient light intensity signal; and

    storing a resultant image in the memory,wherein the one or more programs include instructions for determining a periodic frequency of the ambient light intensity,wherein compensating for ambient light comprises;

    synthesizing or extracting, from one or more received ambient light intensity signals, a complete periodic cycle of ambient light intensity having the determined periodic frequency,extending the ambient light intensity periodic cycle to a time period corresponding to the fluorescence exposure time,calculating a first accumulated ambient light value corresponding to an area under the curve of ambient light intensity during a background exposure time,calculating a second accumulated ambient light value corresponding to an area under the curve of the ambient light intensity during the fluorescence exposure time,scaling the received image sensor output for the background exposure time and the received image sensor output for the fluorescence exposure time based on a ratio of the first and second accumulated ambient light values, andsubtracting the scaled image sensor output for the background exposure time from the scaled image sensor output for the fluorescence exposure time to form the resultant image.

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