Compositions comprising digestive enzymes
First Claim
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1. A composition comprising a recombinant pepsin polypeptide, wherein (a) the composition is free from animal-derived proteins, (b) the pepsin polypeptide is in a proteolytic ally inactive form which does not self-digest, (c) the composition has a pH of 5.4 or higher, and (d) the composition has a specific activity of from 500 FCC units/mg total protein to less than 30,000 FCC units/mg total protein;
- wherein the composition is in powdered form.
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Abstract
Provided herein are compositions with enhanced protein specific activity, protein combinations and methods for the preparation thereof.
135 Citations
23 Claims
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1. A composition comprising a recombinant pepsin polypeptide, wherein (a) the composition is free from animal-derived proteins, (b) the pepsin polypeptide is in a proteolytic ally inactive form which does not self-digest, (c) the composition has a pH of 5.4 or higher, and (d) the composition has a specific activity of from 500 FCC units/mg total protein to less than 30,000 FCC units/mg total protein;
- wherein the composition is in powdered form.
- View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 22, 23)
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15. A method for producing a high-activity stable pepsin powdered composition comprising steps of:
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a. providing a microorganism expressing a recombinant pepsinogen, wherein the expressed pepsinogen is secreted by the microorganism into its growth media; b. harvesting the growth media and removing the microorganismal cells therefrom to obtain a liquid starting material; c. lowering the pH of the liquid starting material to less than pH 4.0 to obtain an activated pepsin composition; d. raising the activated pepsin composition to a pH of 5.4 or higher to obtain a high-activity stable pepsin composition; and e. isolating the stable pepsin polypeptide from protein and small molecules in the liquid starting material to obtain the high-activity stable pepsin powdered composition having a specific activity of less than 10,000 FCC units/mg total protein; wherein step (e) is performed after steps (c) and (d). - View Dependent Claims (16, 17, 18, 19, 20, 21)
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Specification