Device for mechanically characterizing an element of interest such as an oocyte

• US 11,603,516 B2
• Filed: 03/20/2018
• Issued: 03/14/2023
• Est. Priority Date: 03/21/2017
• Status: Active Grant

• Current Assignee: Centre National De La Recherche Scientifique, Ecole Nationale Supérieure de Mécanique et des Microtechniques, Universite De Franche-Comte
• Original Assignee: Centre National De La Recherche Scientifique
• Inventors: Abadie, Joel, Gana, Racha, Piat, Emmanuel
• Primary Examiner(s): Hopkins, Brandi N
• Application Number:
  US16/495,918
• Publication Number:
  US 20200131464A1
• Time in Patent Office: 1,820 Days
• CPC Class Codes: C12M 35/06 Magnetic means C12M35/02 takes precedence
• CPC Class Codes: C12M 41/46 of cellular or enzymatic activity or functionality, e.g. cell viability
• CPC Class Codes: G01N 2203/0078 using indentation
• CPC Class Codes: G01N 2203/0085 Compressibility
• CPC Class Codes: G01N 2203/0087 Resistance to crushing
• CPC Class Codes: G01N 2203/0089 Biorheological properties
• CPC Class Codes: G01N 2203/0286 Miniature specimen; Testing on microregions of a specimen
• CPC Class Codes: G01N 3/40 Investigating hardness or rebound hardness
• CPC Class Codes: G01N 3/42 by performing impressions under a steady load by indentors, e.g. sphere, pyramid G01N3/54 takes precedence

Abstract

Disclosed is a device for mechanically characterizing an element of interest, for example an oocyte. The mechanical characterization device includes: a support receiving a container suitable for containing a liquid medium; a holder for holding the element of interest; an indenting member; a magnet for generating a magnetic field in which the indenting member is intended to move and which participates in suspending the indenting member with an unstable horizontal direction oriented coaxially to the longitudinal axis; a controller to control the magnet to maneuver the indenting member in translation along the unstable horizontal direction; and a component for determining the mechanical characteristics of the element of interest.

Claims

1. A device for mechanically characterizing an element of interest (E), advantageously a microscopic element of interest, possibly biological in nature, or even a cell,

wherein the mechanical characterization device (1) comprises:

support means (2), for receiving a container (C) suitable for containing a liquid medium,

holding means (3), for holding said element of interest (E) in said liquid medium,

an indenting member (4) having a longitudinal axis (4′

) and intended to remain in suspension in said liquid medium, midwater, with said longitudinal axis (4′

) oriented horizontally, wherein said indenting member (4) includes at least one permanent magnet (45) and one proximal end (42) intended to indent said element of interest (E),

magnetic means (5), for generating a magnetic field in which said indenting member (4) is intended to move and which participates in the suspension of said indenting member (4) midwater in the liquid medium of the container (C), and wherein said indenting member (4) is unstable in an unstable horizontal direction (x) directed coaxially to said longitudinal axis (4′

) of said indenting member (4),

control means (6), intended to pilot said magnetic means (5) so as to generate a variation of said magnetic field that is adapted to operate said indenting member (4) in translation along said unstable horizontal direction (x), and

means (7) for determining the mechanical characteristics of said element of interest (E), taking into account the characteristics of said magnetic field and the value (D) of translational displacement of said indenting member (4) along said unstable horizontal direction (x) when said proximal end (42) of said indenting member (4) generates a compression force to said element of interest (E).

2. The device for mechanically characterizing an element of interest (E) according to claim 1, wherein the indenting member (4) includes at least two permanent magnets (45) that are arranged:

with magnetic fields coaxial to each other and in a same direction NS-NS, and

in a horizontal equilibrium position, in which the centre of buoyancy is intended to be merged with the centre of gravity.

3. The device for mechanically characterizing an element of interest (E) according to claim 1, wherein said indenting member (4) comprises a body (41) consisted of a capillary delimiting a sealed chamber (44) that is filled with air and that contains said at least one permanent magnet (45).

4. The device for mechanically characterizing an element of interest (E) according to claim 1, wherein the container (C) comprises a bottom (C1) connected to a lateral wall (C2), a free upper edge (C21) of which delimits an upper opening (C22), for example a Petri dish.

5. The device for mechanically characterizing an element of interest (E) according to claim 1, wherein the magnetic means (5) comprise:

at least two permanent magnets (51, 52) that are arranged coaxially, along a horizontal axis (A), and in the same direction NS-NS, and

means (53) for operating said permanent magnets (51, 52) in translation.

6. The device for mechanically characterizing an element of interest (E) according to claim 5, wherein said at least two permanent magnets (51, 52) are each arranged within an electromagnetic coil (54, 55),

wherein said electromagnetic coils (54, 55) are arranged coaxially along the horizontal axis (A) and are connected to means (56) for piloting the electric current powering said electromagnetic coils (54, 55).

7. The device for mechanically characterizing an element of interest (E) according to claim 5, wherein said at least two permanent magnets (51, 52), and said at least two electromagnetic coils (54, 55), are arranged on either side of the container (C), at a constant distance d from each other.

8. The device for mechanically characterizing an element of interest (E) according to claim 1, wherein the holding means (3) comprise a suction pipette (31).

9. The device for mechanically characterizing an element of interest (E) according to claim 1, wherein the means (7) for determining the mechanical characteristics of said element of interest (E) comprise means (71, 72) for determining the value (D) of translational displacement of the indenting member (4) along said unstable horizontal direction (x).

10. A medically assisted procreation injection station, equipped with a device according to claim 1.

11. A method for studying the mechanical characteristics of an element of interest (E) by implementing a device (1) according to claim 1,

wherein the method comprises:

a) a preparation phase in which, on the one hand, said element of interest (E) is held in the liquid medium of the container (C) by said holding means (3) and, on the other hand, the magnetic means (5) generate an initial magnetic field (P1) that allows holding said indenting member (4) in an initial position at rest, remote from said element of interest (E) and midwater in the liquid medium of the container (C),

b) a loading phase during which the magnetic means (5) are piloted so as to modify the magnetic field from said initial magnetic field (P1) to a modified magnetic field (P2), wherein said indenting member (4) is operated in translation along said unstable horizontal direction (x), in a loading direction in which said proximal end (42) of said indenting member (4), midwater in the liquid medium of the container (C), generates a compression force to said element of interest (E) held by said holding means (3),

c) an unloading phase during which the magnetic means (5) are piloted so as to restore said initial magnetic field (P1) in which said indenting member (4) is operated in translation along said unstable horizontal direction (x), in an unloading direction in which said proximal end (42) of said indenting member (4), midwater in the liquid medium of the container (C), moves apart from said element of interest (E) held by said holding means (3),

said method comprises a step of collecting the value (D) of translational displacement of said indenting member (4) along said unstable horizontal direction (x), at least during the loading phase, and

said method comprises a step of determining mechanical characteristics of said element of interest (E), taking into account the characteristics of said magnetic field (E) and the value (D) of translational displacement of said indenting member (4) along said unstable horizontal direction (x).

12. The method according to claim 11, wherein the magnetic means (5) comprise:

at least two permanent magnets (51, 52) that are arranged coaxially, along a horizontal axis (A), and in the same direction NS-NS, and

means (53) for operating said permanent magnets (51, 52) in translation;

wherein the characteristics of said magnetic field are function of;

the value of displacement of the magnetic means (5) and/or

the value of the electric current injected into the electromagnetic coils (54, 55).

13. The method according to claim 12, wherein the element of interest (E) is a cell chosen among:

the oocytes, or

the cancer cell.

14. The method according to claim 11, wherein the element of interest (E) is a cell chosen among:

the oocytes, or

the cancer cell.

15. The method according to claim 11, wherein the magnetic means (5) comprise:

at least two permanent magnets (51, 52) that are arranged coaxially, along a horizontal axis (A), and in the same direction NS-NS; and

means (53) for operating said permanent magnets (51, 52) in translation;

wherein said at least two permanent magnets (51, 52) are each arranged within an electromagnetic coil (54, 55),

wherein said electromagnetic coils (54, 55) are arranged coaxially along the horizontal axis (A) and are connected to means (56) for piloting the electric current powering said electromagnetic coils (54, 55), and

wherein the characteristics of said magnetic field are function of;

the value of displacement of the magnetic means (5) and/or

the value of the electric current injected into the electromagnetic coils (54, 55).

16. The method according to claim 15, wherein the element of interest (E) is a cell chosen among:

the oocytes, or

the cancer cell.