DNA detection device

• US 20010026917A1
• Filed: 12/28/2000
• Published: 10/04/2001
• Est. Priority Date: 12/28/1999
• Status: Active Grant

• Current Assignee: Fujifilm Corporation (Fujifilm Holdings Corporation)
• Original Assignee: Fuji Photo Film Co Limited (Fujifilm Holdings Corporation)
• Inventors: Ogura, Nobuhiko, Hosoi, Yuichi, Neriishi, Keiko, Umemoto, Chiyuki
• Granted Patent: US 6,861,216 B2
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01J 2219/00527 Sheets
• CPC Class Codes: B01J 2219/00574 radioactive
• CPC Class Codes: B01J 2219/00585 Parallel processes
• CPC Class Codes: B01J 2219/00596 Solid-phase processes
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00608 DNA chips
• CPC Class Codes: B01J 2219/00659 Two-dimensional arrays
• CPC Class Codes: B01J 2219/00707 separated from the reactor apparatus
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: C12Q 1/6837 using probe arrays or probe chips C12Q1/6874 takes precedence
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: G01T 1/2942 using autoradiographic methods

Abstract

A process for detecting a complementary DNA fragment is performed utilizing a DNA micro-array, a radiation image storage panel containing a stimulable phosphor, and a spacer sheet having plural openings.

Claims

1. A process for detecting a complementary DNA fragment which comprises the steps of:

bringing single-stranded sample DNA fragments having a radioactive label in a liquid phase into contact with a DNA micro-array having at least two defined areas in each of which a group of nucleotide derivatives and analogues thereof are fixed under such condition that a group of nucleotide derivatives and analogues thereof fixed in one area differs from a group of nucleotide derivatives and analogues thereof fixed in another area, so that DNA fragments complementary to a group of nucleotide derivatives and analogues thereof are fixed by hybridization to the area in which the group is fixed;

removing unfixed sample DNA fragments from the DNA micro-array;

keeping the DNA micro-array in contact with a radiation image storage panel containing a stimulable phosphor via a spacer sheet having openings in areas corresponding to the areas on which groups of nucleotide derivatives or analogues thereof are fixed, so that the stimulable phosphor sheet can absorb and store radiation energy of the radioactive label coming from the fixed DNA fragments through the openings;

irradiating the radiation image storage panel with a stimulating light, so that the image storage panel releases a stimulated emission from the area in which the radiation energy is stored;

detecting the stimulated emission photoelectrically to obtain a series of electric signals; and

processing the electric signals to locate the area in which the complementary DNA fragments are fixed.

2. The process of

claim 1

, in which the spacer sheet is made of non radiation-transmitting material.

3. The process of

claim 1

, in which the radiation image storage panel is irradiated with a stimulating light after it is separated from the DNA micro-array.

4. A kit for detecting complementary DNA fragments comprising a DNA micro-array having at least two defined areas in each of which a group of nucleotide derivatives and analogues thereof are fixed under such condition that a group of nucleotide derivatives and analogues thereof fixed in one area differs from a group of nucleotide derivatives and analogues thereof fixed in another area, a radiation image storage panel containing a stimulable phosphor, and a spacer sheet having openings in areas corresponding to the areas on which groups of nucleotide derivatives or analogues thereof are fixed.

5. The kit of

claim 4

, in which the spacer sheet is made of non radiation-transmitting material.

6. A composite structure comprising a DNA micro-array having at least two defined areas in each of which a group of nucleotide derivatives and analogues thereof are fixed under such condition that a group of nucleotide derivatives and analogues thereof fixed in one area differs from a group of nucleotide derivatives and analogues thereof fixed in another area, a spacer sheet having openings in areas corresponding to the areas on which groups of nucleotide derivatives or analogues thereof are fixed, and a radiation image storage panel containing a stimulable phosphor, overlaid in order, the spacer sheet being positioned in relation to the DNA micro-array in such condition that the openings of the spacer sheet face the areas of the micro-array in which groups of nucleotide derivatives and analogues thereof are fixed.

7. A radiation image storage panel containing a stimulable phosphor, which has on its surface a spacer film of non radiation-transmitting material having plural openings are formed.