Method for monitoring flow rate using fluorescent markers

  • US 20010027918A1
  • Filed: 01/11/2001
  • Published: 10/11/2001
  • Est. Priority Date: 01/14/2000
  • Status: Active Grant
First Claim
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1. A method of monitoring a flow rate of a fluidic material in a microfluidic device, the method comprising:

  • (i) flowing a first marker moiety through the at least one microscale channel;

    (ii) flowing the fluidic material through the at least one microscale channel;

    (iii) flowing a second marker moiety through the at least one microscale channel;

    (iv) detecting the first marker moiety, resulting in detection of a first signal having, a first area and a first retention time;

    (v) detecting the second marker moiety, resulting in detection of a second signal having a second area and a second retention time; and

    , (vi) deconvoluting the first signal and the second signal to provide an indication of the flow rate of the fluidic material, wherein the deconvoluting comprises identifying differences between two or more of;

    the first signal, the second signal, a first selected standard, or a second selected standard, thereby monitoring the flow rate of the fluidic material.

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