Vaccine composition, method of preparing the same, and method of vaccinating vertebrates

  • US 20020009457A1
  • Filed: 05/11/2001
  • Published: 01/24/2002
  • Est. Priority Date: 05/12/2000
  • Status: Active Grant
First Claim
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1. A method of preparing a vaccine composition, said method comprising the steps of:

  • (A) forming a water-in-oil emulsion comprising (a) water, (b) an alginate, (c) an oil, (d) an antigen, and (e) a surfactant composition comprising at least one of (i) a cellulose ether and at least one nonionic surfactant and (ii) a poly(ethylene oxide)-poly(propylene oxide)-poly(ethylene oxide) triblock copolymer surfactant and at least one nonionic surfactant;

    (B) crosslinking the alginate in the emulsion of step (A) with at least two cations selected from the group consisting of aluminum, barium, calcium, lithium, manganese, strontium, and zinc, to form antigen-containing crosslinked alginate microparticles; and

    (C) harvesting the microparticles of step (B).

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