Assay systems and methods
First Claim
1. A method of detecting a reaction, comprising:
- providing a first reagent mixture which comprises a first reagent having a fluorescent label;
introducing a second reagent into the first reagent mixture to produce a second reagent mixture, the second reagent reacting with the first reagent to produce a fluorescently labeled product having a substantially different charge than the first reagent;
introducing a polyion into at least one of the first and second reagent mixtures; and
comparing a fluorescent polarization in the second reagent mixture relative to the first reagent mixture.
1 Assignment
0 Petitions
Accused Products
Abstract
Methods, systems, kits for carrying out a wide variety of different assays that comprise providing a first reagent mixture which comprises a first reagent having a fluorescent label. A second reagent is introduced into the first reagent mixture to produce a second reagent mixture, where the second reagent reacts with the first reagent to produce a fluorescently labeled product having a substantially different charge than the first reagent. A polyion is introduced into at least one of the first and second reagent mixtures, and the fluorescent polarization in the second reagent mixture relative to the first reagent mixture is determined, this fluorescent polarization being indicative of the rate or extent of the reaction.
-
Citations
1 Claim
-
1. A method of detecting a reaction, comprising:
-
providing a first reagent mixture which comprises a first reagent having a fluorescent label;
introducing a second reagent into the first reagent mixture to produce a second reagent mixture, the second reagent reacting with the first reagent to produce a fluorescently labeled product having a substantially different charge than the first reagent;
introducing a polyion into at least one of the first and second reagent mixtures; and
comparing a fluorescent polarization in the second reagent mixture relative to the first reagent mixture.
-
Specification