SYNTHETIC GENES AND GENETIC CONSTRUCTS COMPRISING SAME I

• US 20020168707A1
• Filed: 06/19/1998
• Published: 11/14/2002
• Est. Priority Date: 03/20/1998
• Status: Active Grant

• Current Assignee: Commonwealth Scientific & Industrial Research Organisation (Commonwealth Of Australia As Represented By Department Of Industry, Innovation, Climate Change, Science, Research And Tertiary Education)
• Original Assignee: Benitec Australia Limited (Benitec Biopharma, Inc.), State Of Queensland Through Its Department Of Primary Industries
• Inventors: GRAHAM, MICHAEL WAYNE
• Granted Patent: US 6,573,099 B2
• Time in Patent Office: Days
• US Class Current: 435/69.1
• CPC Class Codes: C12N 15/113 Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing when used in plants C12N15/8218
• CPC Class Codes: C12N 15/63 Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
• CPC Class Codes: C12N 15/69 Increasing the copy number of the vector
• CPC Class Codes: C12N 15/8216 Methods for controlling, regulating or enhancing expression of transgenes in plant cells
• CPC Class Codes: C12N 15/8218 Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
• CPC Class Codes: C12N 15/8283 for virus resistance
• CPC Class Codes: C12N 15/85 for animal cells
• CPC Class Codes: C12N 2800/108 episomal vectors
• CPC Class Codes: C12N 2830/00 Vector systems having a special element relevant for transcription
• CPC Class Codes: C12N 2830/002 inducible enhancer/promoter combination, e.g. hypoxia, iron, transcription factor
• CPC Class Codes: C12N 2830/15 chimeric enhancer/promoter combination
• CPC Class Codes: C12N 2830/38 being a stuffer
• CPC Class Codes: C12N 2830/42 being an intron or intervening sequence for splicing and/or stability of RNA
• CPC Class Codes: C12N 2830/55 from bacteria
• CPC Class Codes: C12N 2830/60 from viruses
• CPC Class Codes: C12N 2840/102 inhibiting translation
• CPC Class Codes: C12N 2840/20 translation of more than one cistron
• CPC Class Codes: C12N 9/0071 acting on paired donors with incorporation of molecular oxygen (1.14)
• CPC Class Codes: C12N 9/1051 Hexosyltransferases (2.4.1)
• CPC Class Codes: C12N 9/127 RNA-directed RNA polymerase (2.7.7.48), i.e. RNA replicase
• CPC Class Codes: C12N 9/503 : derived from viruses

Abstract

The present invention relates generally to synthetic genes for modifying endogenous gene expression in a cell, tissue or organ of a transgenic organism, in particular a transgenic animal or plant. More particularly, the present invention provides novel synthetic genes and genetic constructs which are capable of repressing delaying or otherwise reducing the expression of an endogenous gene or a target gene in an organism when introduced thereto.

Claims

1. A synoptic gene which is capable of modifying target gene expression in a cell, tissue or organ of a prokaryotic or eukaryotic organism which is transfected or transformed therewith, wherein said synthetic gene at least comprises a structural gene sequence comprising a nucleotide sequence which is substantially identical to the nucleotide sequence of said target gene or a derivative thereof or a complementary sequence thereto placed operably under the control of a promoter sequence which is operable in said cell, tissue or organ.

2. A synthetic gene which is capable of modifying the expression of a target gene in a cell, tissue or organ of a prokaryotic or eukaryotic organism which is transfected or transformed therewith, wherein said synthetic gene at least comprises multiple structural gene sequences, wherein each of said structural gene sequences comprises a nucleotide sequence which is substantially identical to the nucleotide sequence of said target gene or a derivative thereof or a complementary sequence thereto and wherein said multiple structural gene sequences are placed operably under the control of a single promoter sequence which is operable in said cell, tissue or organ.

3. A synthetic gene which is capable of modifying the expression of a target gene in a cell, tissue or organ of a prokaryote or eukaryote which is transfected or transformed therewith wherein said synthetic gene at least comprises multiple structural gene sequences wherein each of said structural gene sequences is placed operably under the control of a promoter sequence which is operable in said cell, tissue or organ and wherein each of said structural gene sequences comprises a nucleotide sequence which is substantially identical to the nucleotide sequence of said target gene or a derivative thereof or a complementary sequence thereto.

4. A genetic construct which is capable of modifying the expression of an endogenous gene or target gene in a transformed or transfected cell, tissue or organ wherein said genetic construct at least comprises the synthetic gene of the invention and one or more origins of replication and/or selectable marker gene sequences.

5. A cell, tissue, organ or organism comprising the synthetic genes and genetic constructs described herein.

6. Plasmid pEGFP-N1 MCS or a derivative thereof.

7. Plasmid pCMV.cass or a derivative thereof.

8. Plasmid pCR.Bgl-GFP-Bam or a derivative thereof.

9. Plasmid pCR.SV40L or a derivative thereof.

10. Plasmid pCMV.SV40L.cass or a derivative thereof.

11. Plasmid pCR.BEV.1 or a derivative thereof.

12. Plasmid pCR.BEV.2 or a derivative thereof.

13. Plasmid pCR.BEV.3 or a derivative thereof.

14. Plasmid pEGFP.BEV.1or a derivative thereof.

15. Plasmid pCMV.BEV.2 or a derivative thereof.

16. Plasmid pCMV.VEB or a derivative thereof.

17. Plasmid pCMV.BEVnt or a derivative thereof.

18. Plasmid pCMV.BEV×

2 or a derivative thereof.

19. Plasmid pCMV.BEV.VEB or a derivative thereof.

20. Plasmid pCMV.BEV.GFP.VEB or a derivative thereof.

21. Plasmid pCMV.SV40L-O or a derivative thereof.

22. Plasmid pCMV.0.SV40L.BEV or a derivative thereof.

23. Plasmid pCMV.0.SV40L.VEB or a derivative thereof.

24. Plasmid pCMV.BEV.SV40L.BEV or a derivative thereof.

25. Plasmid pCMV.BEV.SV40L.VEB or a derivative thereof.

26. Plasmid pCMV.SV40LR.cass or a derivative thereof.

27. Plasmid pCMV.BEV.SV40LR or a derivative thereof.

28. Plasmid pCMV.TYR or a derivative thereof.

29. Plasmid pCMV.TYRLIB or a derivative thereof.

30. Plasmid pCMV.Lac or a derivative thereof.

31. Plasmid pCMVLacI.OPRSV1.cass or a derivative thereof.

32. Plasmid pCMVLacI.OPRSV1.GFP.cass or a derivative thereof.

33. Plasmid pCMVLacI.TYR.OPRSV1.GFP or a derivative thereof.