Rapid assay for arthopod-borne disease vectors and pathogens
First Claim
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1. A method for analyzing an arthropod sample for the presence of one or more analytes associated with the pathogen that causes human malaria, comprising:
- a) contacting a liquid permeable support with the arthropod sample and one or more detectable analyte-specific reagents that bind specifically to a protein analyte associated with a Plasmodium sporozoite, if present, to form analyte-reagent complexes, said support comprising at least one detection area, said area having an analyte-specific capture reagent immobilized therein, said capture reagent specific for the protein analyte associated with the Plasmodium sporozoite, said capture reagent being adapted for capturing the analyte-reagent complexes; and
b) detecting the presence of the detectable analyte-specific reagent in the detection area, indicating the presence of the analyte in the sample.
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Abstract
A method is described for detecting arthropod-borne human pathogens in arthropods using a rapid dipstick format where the signal for one or more pathogens can be visually detected on a single test dipstick. Specific examples of pathogens detected include Plasmodium species, togaviruses, flaviviruses, encephalitis viruses and Ross River virus. These tests are rapid, easy to use and are suited for use in the field.
31 Citations
43 Claims
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1. A method for analyzing an arthropod sample for the presence of one or more analytes associated with the pathogen that causes human malaria, comprising:
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a) contacting a liquid permeable support with the arthropod sample and one or more detectable analyte-specific reagents that bind specifically to a protein analyte associated with a Plasmodium sporozoite, if present, to form analyte-reagent complexes, said support comprising at least one detection area, said area having an analyte-specific capture reagent immobilized therein, said capture reagent specific for the protein analyte associated with the Plasmodium sporozoite, said capture reagent being adapted for capturing the analyte-reagent complexes; and
b) detecting the presence of the detectable analyte-specific reagent in the detection area, indicating the presence of the analyte in the sample. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28)
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14. A method for analyzing an arthropod sample for the presence of at least one analyte associated with at least one type of arthropod-carried agent, wherein the arthropod-carried agent is a togavirus, comprising:
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a) contacting a liquid permeable support with the arthropod sample and a detectable analyte-specific reagent that binds to an analyte associated with the togavirus, if present, to form analyte-reagent complex, said support comprising a detection area, said area having an analyte-specific capture reagent immobilized therein, said capture reagent specific for the analyte associated with the togavirus, said capture reagent being adapted for capturing the analyte-reagent complex; and
b) detecting the presence of the detectable analyte-specific reagent in the detection area, indicating the presence of the analyte in the sample.
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29. A method for analyzing an arthropod sample for the presence of an analyte associated with a Ross River virus arthropod-carried agent, comprising:
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a) contacting a liquid permeable support with the arthropod sample and a detectable analyte-specific reagent that binds to an analyte associated with Ross River virus, if present, to form analyte-reagent complex, said support comprising a detection area, said area having an analyte-specific capture reagent immobilized therein, said capture reagent specific for the analyte associated with Ross River virus, said capture reagent being adapted for capturing the analyte-reagent complex; and
b) detecting the presence of the detectable analyte-specific reagent in the detection area, indicating the presence of the analyte in the sample.
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30. A method for analyzing an arthropod sample for the presence of two or more analytes associated with an arthropod-carried agent, comprising:
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a) contacting a liquid permeable support with the arthropod sample and at least two detectable analyte-specific reagents that bind to each of the analytes, if present, to form analyte-reagent complexes, said support comprising at least two detection areas, said areas each having an analyte-specific capture reagent immobilized therein, said capture reagent being adapted for capturing one of the analyte-reagent complexes; and
b) detecting the presence of the detectable analyte-specific reagent in each of the detection areas, indicating the presence of the analyte in the sample.
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- 31. A kit for analyzing an arthropod sample for the presence or absence of at least one analyte associated with an arthropod-borne agent, comprising a liquid permeable support for contacting with said arthropod sample and at least one detectable analyte-specific reagent that forms an analyte-reagent complex with said analyte, said support comprising at least two detection areas having a capture reagent immobilized therein, said capture reagent being adapted for capturing the analyte-reagent complex.
Specification