Device and electrode arrangement for electrophysiological studies

• US 20030009112A1
• Filed: 08/30/2002
• Published: 01/09/2003
• Est. Priority Date: 03/02/2000
• Status: Abandoned Application

• Current Assignee: NMI Natural and Medical Sciences Institute at the University TÜBINGEN
• Original Assignee: NMI Natural and Medical Sciences Institute at the University TÜBINGEN
• Inventors: Martin, Dieter, Leibrock, Cornelia, Nisch, Wilfried, Hammerle, Hugo
• Application Number:
  US10/234,664
• Publication Number:
  US 20030009112A1
• Time in Patent Office: Days
• US Class Current: 600/547
• CPC Class Codes: A61B 5/145 Measuring characteristics of blood in vivo, e.g. gas concentration, pH value; Measuring characteristics of body fluids or tissues, e.g. interstitial fluid, cerebral tissuemeasuring of blood pressure or blood flow A61B5/02; non-radiation detecting or locating of foreign bodies in blood A61B5/06
• CPC Class Codes: G01N 33/4836 using multielectrode arrays

Abstract

A device is disclosed for electrophysiological studies on biological material. The device comprises a support on which an array of measurement electrodes is arranged. A vessel is provided having a cavity for the biological material and an appropriate culture medium. The vessel is arranged on the support around the measurement electrodes in such a way that the latter are in electrical contact with the cavity. By means of a counter electrode, which is permanently arranged in the cavity, electrical signals can be measured between the measurement electrodes and the counter electrode.

Claims

1. A device for electrophysiological studies on biological material contained in an appropriate culture medium, said device comprising:

a vessel forming a cavity for accommodating said biological material and said culture medium,

a support coupled to said vessel and having an array of measurement electrodes arranged on said support, and

one counter electrode permanently arranged on said support,

wherein said vessel is arranged around said measurement electrodes and said one counter electrode in such a way that said measurement electrodes are adapted to make electrical contact with said biologic material, and

wherein said measurement electrodes and said one counter electrode are configured for measuring electrical signals between said measurement electrodes and said one counter electrode, or for providing an electrical stimulating signal to said biological material.

2. The device of claim 1, wherein said one counter electrode has a counter electrode surface area and wherein any of said measurement electrodes have a measurement electrode surface area, said counter electrode surface area being at least 10³ times larger than any of said measurement electrode surface areas.

3. The device of claim 2, wherein said counter electrode surface area is between about 0.1 mm² and 1 cm².

4. The device of claim 3, wherein said counter electrode surface area is between approximately 10 and 100 mm².

5. The device of claim 1, wherein said one counter electrode is located in immediate proximity to said array.

6. The device of claim 1, further comprising a terminal surface for making electrical contact to said one counter electrode, said terminal surface being arranged on said support outside said cavity.

7. A device for electrophysiological studies on biological material contained in an appropriate culture medium, said device comprising:

a vessel forming a cavity for accommodating said biological material and said culture medium,

a support and an array of measurement electrodes being arranged on said support, and

a counter electrode,

wherein said vessel is arranged on said support and around said measurement electrodes in such a way that said measurement electrodes are adapted to make electrical contact with said biological material,

wherein said measurement electrodes and said counter electrode are configured for measuring electrical signals between said measurement electrodes and said counter electrode, or for providing electrical stimulating signals to said biological material, and

wherein said counter electrode is permanently arranged in said cavity.

8. The device of claim 7, wherein said vessel comprises a circumferential wall and said counter electrode is arranged internally of said vessel and on said circumferential wall.

9. The device of claim 7, further comprising a lid for said vessel, said counter electrode being arranged internally of said vessel and on said lid.

10. The device of claim 7, wherein said counter electrode is arranged on said support.

11. The device of claim 10, wherein said counter electrode is integrated into said support.

12. The device of claim 11, wherein said counter electrode is fabricated in the same technology as said measurement electrodes.

13. The device of claim 10, further comprising, for making contact with said measurement electrodes, a plurality of conductor tracks providing a profile, and said counter electrode surface area comprises a shape which is matched to said profile.

14. The device of claim 10, wherein said counter electrode is located in immediate proximity to said array.

15. The device of claim 7, wherein said counter electrode is fabricated from fractal material having microporous structures.

16. The device of claim 15, wherein said counter electrode is made of at least one of the following:

titanium nitride, iridium or iridium oxide.

17. The device of claim 7, wherein said counter electrode has a counter electrode surface area and any of said measurement electrodes have a measurement electrode surface area, wherein said counter electrode surface area is at least 10³ times larger than any of said measurement electrode surface areas.

18. The device of claim 17, wherein said counter electrode surface area is between about 0.1 mm² and 1 cm².

19. The device of claim 18, wherein said counter electrode surface area is between approximately 10 and 100 mm².

20. A microelectrode arrangement for electrophysiological measurements on biological material, said electrode arrangement comprising:

a support having an array of measurement electrodes integrated on said support, and

a counter electrode permanently integrated on said support,

wherein said measurement electrodes and said counter electrode are configured for measuring electrical signals between said measurement electrodes and said counter electrode, or for providing an electrical stimulating signal to said biological material.

21. The microelectrode arrangement of claim 20, wherein said counter electrode has a counter electrode surface area and wherein any of said measurement electrodes have a measurement electrode surface area, said counter electrode surface area being at least 10³ times larger than any of said measurement electrode surface areas.

22. The microelectrode arrangement of claim 21, wherein said counter electrode surface area is between about 0.1 mm² and 1 cm².

23. The microelectrode arrangement of claim 22, wherein said counter electrode surface area is between approximately 10 and 100 mm².

24. The microelectrode arrangement of claim 20, wherein said counter electrode is located in immediate proximity to said array.

25. The microelectrode arrangement of claim 20, wherein said counter electrode is fabricated in the same technology as said measurement electrodes.

26. The microelectrode arrangement of claim 20, wherein said counter electrode is made from at least one of the following:

titanium nitride, iridium, iridium oxide.

27. The microelectrode arrangement of claim 20, further comprising, for making contact with said measurement electrodes, a plurality of conductor tracks providing a profile, and said counter electrode surface area comprises a shape which is matched to said profile.

29. The method of claim 28, further comprising the step of providing electrical stimulating signals to said biological material via at least some of said microelectrodes.

28. A method of electrophysiologically studying biological material, said method comprising the steps of

providing a device having a vessel arranged on a support for forming a cavity, an array of microelectrodes being arranged on said support in said cavity, and a counter electrode permanently arranged in said cavity,

introducing a culture medium including said biological material into said cavity, and

measuring electrical signals between said measurement electrodes and said counter electrode.