Enzymatic detergent compositions

• US 20030050211A1
• Filed: 05/20/2002
• Published: 03/13/2003
• Est. Priority Date: 12/14/2000
• Status: Abandoned Application

• Current Assignee: Unilever Home & Personal Care USA
• Original Assignee: Unilever Home & Personal Care Usa Division Of Conopco, Inc.
• Inventors: Van Der Waal, Patrick, Bae-Lee, MyongSuk, Ehrnsperger, Eric Charles, Klugkist, Jan, Hage, Ronald, Swarthoff, Ton
• Application Number:
  US10/151,628
• Publication Number:
  US 20030050211A1
• Time in Patent Office: Days
• US Class Current: 510/305
• CPC Class Codes: C11D 3/28 Heterocyclic compounds containing nitrogen in the ring
• CPC Class Codes: C11D 3/38627 containing lipase
• CPC Class Codes: C11D 3/3932 Inorganic compounds or complexes

Abstract

There is provided an enzymatic detergent composition which comprises:

(a) surfactant;

(b) 10-20,000 LU per gram of the detergent composition of a lipolytic enzyme obtainable from Humicola lanuginosa, Pseudomonas pseudoalcaligenes, Rhizomucor miehei and

(c) a non-cross-bridged polydentate N-donor ligand capable of forming a complex with a transition metal, wherein said complex is capable of catalysing the bleaching of stains on fabrics by means of atmospheric oxygen.

Claims

1. An enzymatic detergent composition which comprises:

(a) surfactant;

(b) 10-20,000 LU per gram of the detergent composition of a lipolytic enzyme obtainable from Humicola lanuginosa, Pseudomonas pseudoalcaligenes, Rhizomucor miehei and

(c) a non-cross-bridged polydentate N-donor ligand capable of forming a complex with a transition metal, wherein said complex is capable of catalysing the bleaching of stains on fabrics by means of atmospheric oxygen.

2. A detergent composition according to claim 1, wherein the lipase is selected from the group consisting of Lipolase, Lipolase ultra, LipoPrime, Lipomax, Liposam, and Lipex.

3. A detergent composition according to any one of the preceding claims, wherein the ligand is defined by the general formula (I) or an transition metal complex thereof, preferably iron, manganese, copper or cobalt,

4. A detergent composition according to claim 3, wherein the Z1, Z2 and Z4 independently represent groups selected from optionally substituted pyridin-2-yl, optionally substituted imidazol-2-yl, optionally substituted imidazol-4-yl, optionally substituted pyrazol-1-yl, and optionally substituted quinolin-2-yl.

5. A detergent composition according to claim 3, wherein Z1, Z2 and Z4 each represent optionally substituted pyridin-2-yl.

6. A detergent composition according to claim 3, wherein the Z1 groups represent identical groups.

7. A detergent composition according to claim 3, wherein each Q3 represents a covalent bond or C1-C4-alkylene, preferably a covalent bond.

8. A detergent composition according to claim 3, wherein T represents hydrogen, hydroxy, methyl, ethyl, benzyl, or methoxy.

9. A detergent composition according to claim 3, wherein Z2 represents an optionally substituted heterocyclic ring or an optionally substituted heteroaromatic ring selected from pyridine, pyrimidine, pyrazine, pyrazole, imidazole, benzimidazole, quinoline, quinoxaline, triazole, isoquinoline, carbazole, indole, isoindole, oxazole and thiazole, preferably optionally substituted pyridin-2-yl or optionally substituted benzimidazol-2-yl, and wherein Z4 represents an optionally substituted heterocyclic ring or an optionally substituted heteroaromatic ring selected from pyridine, pyrimidine, pyrazine, pyrazole, imidazole, benzimidazole, quinoline, quinoxaline, triazole, isoquinoline, carbazole, indole, isoindole, oxazole and thiazole, preferably optionally substituted pyridin-2-yl, or an non-coordinating group selected from hydrogen, hydroxy, alkoxy, alkyl, alkenyl, cycloalkyl, aryl, or benzyl.

10. A detergent composition according to claim 1, wherein the ligand is selected from:

1,1-bis(pyridin-2-yl)-N-methyl-N-(pyridin-2-ylmethyl)methylamine;

N,N-bis(pyridin-2-yl-methyl)-bis(pyridin-2-yl)methylamine, hereafter referred to as N4Py.

N,N-bis(pyridin-2-yl-methyl)-1,1-bis(pyridin-2-yl)-1-aminoethane, hereafter referred to as MeN4Py,

N,N-bis(pyridin-2-yl-methyl)-1,1-bis(pyridin-2-yl)-2-phenyl-1-aminoethane, hereafter referred to as MeN4Py

1,1-bis(pyridin-2-yl)-N,N-bis(6-methyl-pyridin-2-ylmethyl)methylamine;

1,1-bis(pyridin-2-yl)-N,N-bis(5-carboxymethyl-pyridin-2-ylmethyl)methylamine;

1,1-bis(pyridin-2-yl)-1-benzyl-N,N-bis(pyridin-2-ylmethyl)methylamine; and

1,1-bis(pyridin-2yl)-N,N-bis(benzimidazol-2-ylmethyl)methylamine,

wherein -Py represents pyridin-2-yl,

wherein -Py represents pyridin-2-yl.

11. A detergent composition according to claim 1, in which the ligand has the general formula:

12. A detergent composition according to claim 11, wherein:

Q is defined such that a=b=0, c=2 or 3 and n=1;

R₁, R₂, R₃, R₄ each independently represent a coordinating group selected from optionally substituted pyridin-2-yl, optionally substituted imidazol-2-yl, optionally substituted imidazol-4-yl, optionally substituted pyrazol-1-yl, and optionally substituted quinolin-2-yl.

13. A detergent composition according to claim 11, wherein:

R₁, R₂, R₃ each independently represent a coordinating group selected from carboxylate, amido, —

NH—

C(NH)NH₂, hydroxyphenyl, an optionally substituted heterocyclic ring or an optionally substituted heteroaromatic ring selected from pyridine, pyrimidine, pyrazine, pyrazole, imidazole, benzimidazole, quinoline, quinoxaline, triazole, isoquinoline, carbazole, indole, isoindole, oxazole and thiazole; and

R₄ represents a group selected from hydrogen, C_1-20 optionally substituted alkyl, C_1-20 optionally substituted arylalkyl, aryl, and C_1-20 optionally substituted NR₃⁺ (wherein R=C_1-8-alkyl).

14. A detergent composition according to claim 11, wherein:

Q is defined such that a=b=0, c=2 or 3 and n=1;

R₁, R₂, R₃ each independently represent a coordinating group selected from optionally substituted pyridin-2-yl, optionally substituted imidazol-2-yl, optionally substituted imidazol-4-yl, optionally substituted pyrazol-1-yl, and optionally substituted quinolin-2-yl; and

R₄ represents a group selected from hydrogen, C_1-10 optionally substituted alkyl, C_1-5-furanyl, C_1-5 optionally substituted benzylalkyl, benzyl, C_1-5 optionally substituted alkoxy, and C_1-20 optionally substituted N⁺Me₃.

15. A detergent composition according to claim 11, wherein:

R₁, R₄ each independently represent a coordinating group selected from carboxylate, amido, —

NH—

C(NH)NH₂, hydroxyphenyl, an optionally substituted heterocyclic ring or an optionally substituted heteroaromatic ring selected from pyridine, pyrimidine, pyrazine, pyrazole, imidazole, benzimidazole, quinoline, quinoxaline, triazole, isoquinoline, carbazole, indole, isoindole, oxazole and thiazole; and

R₂, R₃ each independently represent a group selected from hydrogen, C_1-20 optionally substituted alkyl, C_1-20 optionally substituted arylalkyl, aryl, and C_1-20 optionally substituted NR₃⁺ (wherein R=C_1-8-alkyl).

16. A detergent composition according to claim 11, wherein:

Q is defined such that a=b=0, c=2 or 3 and n=1;

R₁, R₄ each independently represent a coordinating group selected from optionally substituted pyridin-2-yl, optionally substituted imidazol-2-yl, optionally substituted imidazol-4-yl, optionally substituted pyrazol-1-yl, and optionally substituted quinolin-2-yl; and

R₂, R₃ each independently represent a group selected from hydrogen, C_1-10 optionally substituted alkyl, C_1-5-furanyl, C_1-5 optionally substituted benzylalkyl, benzyl, C_1-5 optionally substituted alkoxy, and C_1-20 optionally substituted N⁺Me₃.

17. A detergent composition according to claim 1, wherein the ligand has the general formula (III), or its protonated or deprotonated analogue:

18. A detergent composition according to claim 17, wherein two of R₁, R₂ each independently represent a coordinating group selected from optionally substituted pyridin-2-yl, optionally substituted imidazol-2-yl, optionally substituted imidazol-4-yl, optionally substituted pyrazol-1-yl, and optionally substituted quinolin-2-yl and R3 represents hydrogen, —

CH₂—

, —

CH₂CH₂—

, or benzyl; and

Q₁ and Q2 represent a group selected from —

CH₂— and

—

CH₂CH₂—

; and

Q represents —

CH₂CH₂—

.

19. A detergent composition according to claim 17, wherein R1, R2, R3 each independently represent a coordinating group selected from optionally substituted pyridin-2-yl, optionally substituted imidazol-2-yl, optionally substituted imidazol-4-yl, optionally substituted pyrazol-1-yl, and optionally substituted quinolin-2-yl;

and Q1=Q2=Q3=—

CH2—

.

20. A detergent composition according to claim 17, wherein the ligand is selected from:

21. A detergent composition according to claim 1, wherein the ligand has the general formula (IV), or its protonated or deprotonated analogue:

22. A detergent composition according to claim 21, wherein Z₁, Z₂ and Z₃ independently represent a coordinating group selected from optionally substituted pyridin-2-yl, optionally substituted imidazol-2-yl, optionally substituted imidazol-4-yl, optionally substituted pyrazol-1-yl, and optionally substituted quinolin-2-yl;

and Q1, Q2, Q3 each represent a group selected from —

CH₂— and

—

CH₂CH₂—

.

23. A detergent composition according to claim 21, wherein Z₁, Z₂ and Z₃ each represent optionally substituted pyridin-2-yl and Q1, Q2, Q3 each represent a group selected from —

CH₂— and

—

CH₂CH₂—

.

24. A bleaching composition according to claim 21, wherein the ligand is selected from tris(pyridin-2-ylmethyl)amine, tris(3-methyl-pyridin-2-ylmethyl)amine, tris(5-methyl-pyridin-2-ylmethyl)amine, and tris(6-methyl-pyridin-2-ylmethyl)amine.

25. A detergent composition according to claim 1, wherein the ligand has the general formula:

26. A detergent composition according to claim 25, wherein Y2 is a substituted or unsubstituted benzene ring having halogen, alkyl or alkoxy substituents thereon;

and wherein Y3 and Y4 are each zero; and

wherein Y1 is one carbon containing node C(R)2 and R is methyl or ethyl.

27. A detergent composition according to claim 25, wherein a unit dose provides an aqueous concentration of air bleaching catalyst in the range 0.1 to 10 μ

M and a concentration of lipase in the range 0.01-10 KLU/1.

28. A detergent composition according to claim 1 wherein the lipase is a polypeptide having an amino acid sequence which:

(a) has at least 90% identity with the wide-type 25 lipase derived from Humicola lanuginosa strain DSM 4109;

(b) compared to said wid-type lipase, comprises a substitution of an electrically neutral or negatively charged amino acid at the surface of the three-dimensional structure within 15 A of E1 or Q249 with a positively charged amino acid; and

(c) comprises a peptide addition at the C-terminal; and

/or

(d) meets the following limitations;

i) comprises a negative amino acid in position E210 of said wild-type lipase;

ii) comprises a negatively charged amino acid in the region corresponding to positions 9-101 of said wild-type lipase; and

iii) comprises a neutral or negative amino acid at a position corresponding to N94 or said wid-type lipase and/or has a negative or neutral net electric charge in the region corresponding to positions 90-101 of said wild-type lipase.