Spatial light modulator apparatus

• US 20030086145A1
• Filed: 07/09/2002
• Published: 05/08/2003
• Est. Priority Date: 11/08/2001
• Status: Active Grant

• Current Assignee: Andor Technology PLC (Oxford Instruments PLC)
• Original Assignee: Imaginative Optics, Inc. (Oxford Instruments PLC)
• Inventors: DeSimone, Andrew Frank, Crary, Bruce
• Granted Patent: US 6,885,492 B2
• Time in Patent Office: Days
• US Class Current: 359/290
• CPC Class Codes: G01N 21/6458 Fluorescence microscopy fluorescence microscopes per se G02B21/0076 and G02B21/16
• CPC Class Codes: G02B 21/06 Means for illuminating specimens
• CPC Class Codes: G02B 21/088 for both incident illumination and transillumination
• CPC Class Codes: G02B 21/365 Control or image processing arrangements for digital or video microscopes G02B21/361, G02B21/362 take precedence
• CPC Class Codes: G09G 2300/0857 Static memory circuit, e.g. flip-flop
• CPC Class Codes: G09G 3/346 based on modulation of the reflection angle, e.g. micromirrors micromirrors devices per se G02B26/0833

Abstract

A spatial light modulator apparatus for an optical microscope system including an optical head with a mounting flange for mounting the optical head to a port of the microscope, a DMD for generating a pattern image of light, a light source mount for receiving a source of illumination, and one or more optical elements for directing light from the source of illumination to the DMD and to direct the pattern image generated by the DMD to the microscope. A DMD controller has a digital input and is connected to the DMD for driving the individual micromirrors of the DMD to generate the pattern image. A pattern generation subsystem is configured to output pattern image data and a digital interface is connected between the digital input of the DMD controller and the pattern generation subsystem, the digital interface configured to provide a digital drive signal to the DMD controller corresponding to the pattern image data.

Claims

1. A spatial light modulator apparatus for an optical microscope system, the apparatus comprising:

an optical head including;

a mounting flange for mounting the optical head to a port of the microscope,

a DMD for generating a pattern image of light,

a light source mount for receiving a source of illumination, and

one or more optical elements for directing light from the source of illumination to the DMD and to direct the pattern image generated by the DMD to the microscope;

a DMD controller having a digital input and connected to the DMD for driving the individual micromirrors of the DMD to generate the pattern image;

a pattern generation subsystem configured to output pattern image data; and

a digital interface connected between the digital input of the DMD controller and the pattern generation subsystem, the digital interface configured to provide a digital drive signal to the DMD controller corresponding to the pattern image data.

2. The apparatus of claim 1 in which the DMD controller is mounted on the optical head adjacent the DMD.

3. The apparatus of claim 1 in which the digital interface is housed on a PC card received in a computer and wherein the pattern generation subsystem is operable on the computer.

4. The apparatus of claim 1 in which the optical head includes a housing with an illumination axis, the DMD is located on the housing on the illumination axis thereof and the mounting flange is located on the housing on the illumination axis thereof opposite the DMD.

5. The apparatus of claim 4 in which the light source mount is located on the optical head housing on an axis transverse to the illumination axis.

6. The apparatus of claim 1 in which the optical head further includes a light baffle positioned such that when the micromirrors of the DMD are in the off state, light reflected by the DMD is directed to the light baffle.

7. The apparatus of claim 1 in which the optical microscope has a field plane and the DMD and the optical elements of the optical head are configured to direct the pattern image generated by the DMD to the field plane of the optical microscope.

8. The apparatus of claim 1 in which the optical microscope has a field plane and the DMD and the optical elements of the optical head are configured to direct the pattern image generated by the DMD to a conjugate of the field plane of the optical microscope.

9. The apparatus of claim 1 further including a camera attached to the microscope, the camera having a field of view and wherein the optical elements are configured such that the DMD pattern image fills part or all of the field of view of the camera.

10. The apparatus of claim 1 further including a light source disposed in the light source mount.

11. The apparatus of claim 10 in which the light source is a halogen lamp, a flash lamp, an arc lamp, or a laser light source.

12. The apparatus of claim 1 in which the light source mount further includes a shutter assembly.

13. The apparatus of claim 1 in which the light source mount further includes a filter holder assembly.

14. The apparatus of claim 1 in which the optical head further includes a secondary light source mount.

15. The apparatus of claim 14 in which the secondary light source mount further includes a shutter assembly.

16. The apparatus of claim 14 in which the secondary light source mount further includes a filter holder assembly.

17. The apparatus of claim 1 in which the optical head further includes a beam splitter mount.

18. The apparatus of claim 1 in which the digital interface includes:

a clock which provides a clock signal; and

a logic device responsive to the pattern image data and the clock signal and configured to assign pixels to the pattern image data and to serialize the assigned pixels according to the clock signal to reformat the pattern image data to correspond to the spatial addressing of the DMD.

19. The apparatus of claim 18 in which the logic device is a programmable logic device or a field programmable gate array.

20. The apparatus of claim 18 further including a digital cable connecting the digital interface to the digital input of the DMD controller.

21. The apparatus of claim 18 in which the DMD controller is configured to buffer the reformatted mask image data and to load the memory cells of the DMD.

22. The apparatus of claim 18 in which the logic device is further configured to generate a plurality of timing signals based on the clock signal to synchronize serialization of the assigned pixels.

23. The apparatus of claim 22 in which the plurality of timing signals correspond to DMD address counter signals.

24. The apparatus of claim 23 in which several DMD address counter signals are multiplied in frequency by the logic device to provide faster global dark resets of the mirrors of the DMD.

25. The apparatus of claim 24 in which the logic device is further configured to provide a reset command, a new state command, and a hold command to reset, tilt to new state, or hold all of the mirrors of the DMD simultaneously.

26. The apparatus of claim 1 in which the optical microscope includes a camera for imaging the specimen viewed by the microscope live on a display.

27. The apparatus of claim 26 in which the pattern generation subsystem configured to output pattern image data comprises:

a drawing editor responsive to an input device for drawing a pattern shape; and

an alpha blending routine responsive to the camera and the drawing editor for representing the drawn pattern shape translucently on the display over the specimen image.

28. The apparatus of claim 27 in which the digital interface is responsive to the drawing editor to provide a digital drive signal which controls the DMD to generate the pattern image.

29. The apparatus of claim 27 in which said pattern generation subsystem further includes a set of stored calibration values and a spatial scale and offset routine interposed between the drawing editor and the digital interface and responsive to the stored calibration values for correlating the pixels of the drawn pattern shape to the pixels of the DMD.

30. An optical head for a spatial light modulator system, the optical head comprising:

a mounting flange for mounting the optical head to a port of the microscope;

a spatial light modulator device for generating a pattern image of light;

a light source mount for receiving a source of illumination;

one or more optical elements for directing light from the source of illumination to the spatial light modulator device and to direct the pattern image generated by the spatial light modulator device to a microscope; and

a controller connected to the spatial light modulator device for driving the spatial light modulator device to generate the pattern image, the controller having a digital input responsive to a digital drive signal.

31. The optical head of claim 30 in which the spatial light modulator device is a DMD.

32. The optical head of claim 30 in which the optical head includes a housing with an illumination axis, the DMD is located on the housing on the illumination axis thereof and the mounting flange is located on the housing on the illumination axis opposite the DMD.

33. The optical head of claim 32 in which the light source mount is located on the optical head housing on an axis transverse to the illumination axis.

34. The optical head of claim 31 in which the optical head further includes a light baffle positioned such that when the micromirrors of the DMD are in the off state, light reflected by the DMD is directed to the light baffle.

35. The optical head of claim 30 in which the optical microscope has a field plane and the spatial light modulator device and the optical elements are configured to direct the pattern image generated by the spatial light modulator device to the field plane.

36. The optical head of claim 30 in which the optical microscope has a field plane and the spatial light modulator device and the optical elements are configured to direct the pattern image generated by the spatial light modulator device to a conjugate of the field plane.

37. The optical head of claim 30 further including a camera attached to the microscope having a field of view and wherein the optical elements of the optical head are configured such that the pattern image generated by the spatial light modulator device fills the field of view of the camera.

38. The optical head of claim 30 further including a light source disposed in the light source mount.

39. The optical head of claim 38 in which the light source is a halogen lamp, an arc lamp, a flash lamp, or a laser light source.

40. The optical head of claim 30 in which the light source mount further includes a shutter assembly.

41. The optical head of claim 30 in which the light source mount further includes a filter holder assembly.

42. The optical head of claim 30 further including a secondary light source mount.

43. The optical head of claim 42 in which the secondary light source mount includes a shutter assembly.

44. The optical head of claim 42 in which the secondary light source mount includes a filter holder assembly.

45. The optical head of claim 30 further including a beam splitter mount.

46. A digital interface interconnected between a controller for a spatial light modulator device and a pattern generation subsystem, the digital interface comprising:

a clock which provides a clock signal; and

a logic device responsive to pattern image data output by the pattern generation subsystem and the clock signal and configured to assign pixels to the pattern image data and to serialize the assigned pixels according to the clock signal to reformat the pattern image data to correspond to the spatial addressing of the spatial light modulator device.

47. The digital interface of claim 46 in which the spatial light modulator is a DMD.

48. The digital interface of claim 46 in which the logic device is a programmable logic device or a field programmable gate array.

49. The digital interface of claim 47 further including a digital cable connecting the digital interface to a DMD controller connected to the DMD.

50. The digital interface of claim 49 in which the DMD controller is configured to buffer the reformatted pattern image data and to load the memory cells of the DMD.

51. The digital interface of claim 46 in which the logic device is further configured to generate a plurality of timing signals based on the clock signal to synchronize serialization of the assigned pixels.

52. The digital interface of claim 51 in which the plurality of timing signals correspond to DMD address counter signals.

53. The digital interface of claim 52 in which several of the said DMD address counter signals are multiplied in frequency by the logic device to provide faster global dark resets.

54. The digital interface of claim 53 in which the logic device is further configured to provide a reset command, a new state command, and a hold command.

55. The digital interface of claim 46 in which the digital interface is on a PC card received in a computer and wherein the pattern generation subsystem is operable on the computer.

56. A pattern generation subsystem configured to output a pattern image data to a spatial light modulator, the pattern generation subsystem comprising:

a drawing editor responsive to an input device for drawing a pattern shape; and

an alpha blending routine responsive to a camera and the drawing editor for representing the drawn pattern shape translucently on a display over a specimen image.

57. The pattern generation subsystem of claim 56 in which the spatial light modulator is a DMD.

58. The pattern generation subsystem of claim 56 further including a digital interface responsive to the drawing editor to provide a digital drive signal which controls the spatial light modulator to generate the pattern image.

59. The pattern generation subsystem of claim 58 further including a set of stored calibration values and a spatial scale and offset routine interposed between the drawing editor and the digital interface and responsive to the stored calibration values for correlating the pixels of the drawn pattern shape to the pixels of the spatial light modulator.

60. A spatial light modulator system for an optical microscope, the system comprising:

an optical head including;

a mounting flange for mounting the optical head to either the reflected light port or the transmitted light port of the microscope,

a spatial light modulator for generating a pattern image of light,

a light source mount for receiving a source of illumination, and

one or more optical elements for directing light from the source of illumination to the spatial light modulator and to direct the pattern image generated by the spatial light modulator to the microscope, and

a controller mounted on the optical head adjacent the spatial light modulator and having a digital input; and

a computer including;

a pattern generation subsystem configured to output pattern image data, and

a digital interface PC card configured to provide a digital drive signal to the controller corresponding to the pattern image data generated by the pattern generation subsystem.

61. A spatial light modulator apparatus for an optical microscope system, the apparatus comprising:

an optical head including;

a mounting flange for mounting the optical head to either the reflected light port or the transmitted light port of the microscope,

a DMD for generating a pattern image of light,

a light source mount for receiving a source of illumination, and

one or more optical elements for directing light from the source of illumination to the DMD and to direct the pattern image generated by the DMD to the microscope;

a DMD controller having a digital input and connected to the DMD for driving the individual micromirrors of the DMD to generate the pattern image;

a pattern generation subsystem configured to output pattern image data; and

a digital interface connected between the digital input of the DMD controller and the pattern generation subsystem, the digital interface including;

a digital output connected to the DMD controller,

a clock which provides a clock signal, and

a logic device responsive to the pattern image data and the clock signal and configured to assign pixels to the pattern image data and to serialize the assigned pixels according to the clock signal to reformat the mask image data to correspond to the spatial addressing of the DMD.

62. A spatial light modulator system for an optical microscope, the system comprising:

an optical head including;

a mounting flange for mounting the optical head to either the reflected light port or the transmitted light port of the microscope,

a DMD for generating a pattern image of light,

a light source mount for receiving a source of illumination, and

one or more optical elements for directing light from the source of illumination to the DMD and to direct the pattern image generated by the DMD to the microscope;

a DMD controller having a digital input and connected to the DMD for driving the individual micromirrors of the DMD to generate the pattern image;

a pattern generation subsystem configured to output pattern image data and including;

a drawing editor responsive to an input device for drawing a pattern shape, and

an alpha blending routine responsive to a camera and the drawing editor for representing the drawn pattern shape translucently on a display over the specimen image; and

a digital interface configured to provide a digital drive signal to the DMD controller corresponding to the pattern image data.

63. A spatial light modulator system for an optical microscope, the system comprising:

an optical head including;

a mounting flange for mounting the optical head to either the reflected light port or the transmitted light port of the microscope,

a spatial light modulator for generating a pattern image of light,

a light source mount for receiving a source of illumination, and

one or more optical elements for directing light from the source of illumination to the spatial light modulator and to direct the pattern image generated by the spatial light modulator to the microscope;

a controller having a digital input and connected to the spatial light modulator for driving the spatial light modulator to generate the pattern image;

a pattern generation subsystem configured to output pattern image data, the pattern generation subsystem including;

a drawing editor responsive to an input device for drawing a pattern shape, and

an alpha blending routine responsive to a camera and the drawing editor for representing the drawn pattern shape translucently on the display over the specimen image; and

a digital interface connected between the digital input of the controller and the pattern generation subsystem, the digital interface configured to provide a digital drive signal to the controller corresponding to the pattern image data, the digital interface including;

a clock which provides a clock signal, and

a logic device responsive to the pattern image data and the clock signal and configured to assign pixels to the pattern image data and to serialize the assigned pixels according to the clock signal to reformat the mask image data to correspond to the spatial addressing of the spatial light modulator.