Schizochytrium PKS genes
First Claim
1. An isolated nucleic acid comprising:
- a Vibrio marinus nucleotide sequence selected from the group consisting of ORF 6 (SEQ ID NO;
77), ORF 7 (SEQ ID NO;
78), ORF 8 (SEQ ID NO;
79), and ORF 9 (SEQ ID NO;
80), as shown in FIG. 6.
6 Assignments
0 Petitions
Accused Products
Abstract
The present invention relates to compositions and methods for preparing poly-unsaturated long chain fatty acids in plants, plant parts and plant cells, such as leaves, roots, fruits and seeds. Nucleic acid sequences and constructs encoding PKS-like genes required for the poly-unsaturated long chain fatty acid production, including the genes responsible for eicosapentenoic acid production of Shewanella putrefaciens and novel genes associated with the production of docosahexenoic acid in Vibrio marinus are used to generate transgenic plants, plant parts and cells which contain and express one or more transgenes encoding one or more of the PKS-like genes associated with such long chain polyunsaturated fatty acid production. Expression of the PKS-like genes in the plant system permits the large scale production of poly-unsaturated long chain fatty acids such as eicosapentenoic acid and docosahexenoic acid for modification of the fatty acid profile of plants, plant parts and tissues. Manipulation of the fatty acid profiles allows for the production of commercial quantities of novel plant oils and products.
73 Citations
40 Claims
-
1. An isolated nucleic acid comprising:
a Vibrio marinus nucleotide sequence selected from the group consisting of ORF 6 (SEQ ID NO;
77), ORF 7 (SEQ ID NO;
78), ORF 8 (SEQ ID NO;
79), and ORF 9 (SEQ ID NO;
80), as shown in FIG. 6.
-
2. An isolated nucleic acid comprising:
a nucleotide sequence which encodes a polypeptide of a polyketide-like synthesis system, wherein said system produces a docosahexenoic acid when expressed in a host cell. - View Dependent Claims (3, 4, 5)
-
6. An isolated nucleic acid comprising a Schizochytrium nucleotide sequence comprising a sequence shown in a SEQ ID NO selected from the group consisting of SEQ ID NOS:
- 69, 71 and 76.
- View Dependent Claims (8, 9, 10, 11, 12, 13, 14, 15, 16)
-
7. An isolated nucleic acid comprising:
a nucleotide sequence which is substantially identical to a sequence of at least 50 nucleotides of a Vibrio marinus nucleotide sequence selected from the group consisting of ORF 6 (SEQ ID NO;
77), ORF 7 (SEQ ID NO;
78), ORF 8 (SEQ ID NO;
79), and ORF 9 (SEQ ID NO;
80), as shown in FIG. 6.
-
17. A method for production of docosahexenoic acid in a microbial cell culture, said method comprising:
growing a microbial cell culture having a plurality of microbial cells, wherein said microbial cells or ancestors of said microbial cells were transformed with a vector comprising one or more nucleic acids having a nucleotide sequence which encodes a polypeptide of a polyketide synthesizing system, wherein said one or more nucleic acids are operably linked to a promoter, under conditions whereby said one or more nucleic acids are expressed and docosahexenoic acid is produced in said microbial cell culture. - View Dependent Claims (19, 21)
-
18. A method for production of a long chain polyunsaturated fatty acid in a plant cell, said method comprising:
growing a plant having a plurality of plant cells, wherein said plant cells or ancestors of said plant cells were transformed with a vector comprising one or more nucleic acids having a nucleotide sequence which encodes one or more polypeptides of a polyketide synthesizing system which produces a long chain polyunsaturated fatty acid, wherein each of said nucleic acids are operably linked to a promoter functional in a plant cell, under conditions whereby said polypeptides are expressed and a long chain polyunsaturated fatty acid is produced in said plant cells. - View Dependent Claims (20, 22, 23, 24)
-
25. A recombinant plant cell comprising:
one or more nucleic acids having a nucleotide sequence which encodes one or more polypeptides of a polyketide synthesizing system which produces a long chain polyunsaturated fatty acid, wherein each of said nucleic acids are operably linked to a promoter functional in said plant cell. - View Dependent Claims (26, 27, 28, 29, 30, 31, 32, 33, 34)
-
35. A recombinant E. coli cell comprising:
one or more nucleic acids having a nucleotide sequence which encodes one or more polypeptides of a polyketide synthesizing system which produces a long chain polyunsaturated fatty acid, wherein each of said nucleic acids are operably linked to a promoter function in said E. coli cell. - View Dependent Claims (36, 37)
-
38. A plant oil produced by a recombinant plant cell wherein said plant oil comprises a long chain polyunsaturated fatty acid exogenous to said plant oil, wherein said plant cell is produced according to a method comprising:
transforming said plant cell or an ancestor of said plant cell with a vector comprising one or more polypeptide of a polyketide synthesizing system which produces a long chain polyunsaturated fatty acid wherein each of said nucleic acids are operably linked to a promoter functional in said plant cell. - View Dependent Claims (39, 40)
Specification