Detection of microsporidial species using a quantitative real-time PCR assay

  • US 20030215811A1
  • Filed: 05/17/2002
  • Published: 11/20/2003
  • Est. Priority Date: 05/17/2002
  • Status: Abandoned Application
First Claim
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1. A method for detecting microsporidial species in samples comprising:

  • a. degrading a dual-fluorescently labeled DNA hybridization probe and a set of species-specific primers during PCR amplification of regions in a gene located within the spores of interest; and

    b. reading the fluorescent labels during the PCR process.

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