Optical bio-discs and fluidic circuits for analysis of cells and methods relating thereto
First Claim
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1. An optical bio-disc for conducting a cellular assay, said disc comprising:
- a rotatable substrate;
a cap portion associated with said substrate; and
a fluidic circuit formed between said substrate and said cap portion, said fluidic circuit comprising;
a mixing chamber, said mixing chamber including an inlet port;
a purification chamber in fluid communication with said mixing chamber;
a filter means associated with said purification chamber;
an analysis chamber in fluid communication with said purification chamber, said analysis chamber including a capture zone; and
a vent port associated with said analysis chamber.
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Abstract
The present invention is directed to optical bio-discs, methods, and optical disc drives for clinical diagnostics. The present invention is further directed to methods for determining the quantity of a specific type of blood cell in a biological sample including on-disc sample preparation and processing, providing a pre-processed sample onto a capture zone, removing portions of the sample that are not bound in the capture zone, and counting bound cells. Also disclosed are fluidic circuits for processing blood samples for use in a cluster designation marker assay in the optical bio-disc.
153 Citations
27 Claims
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1. An optical bio-disc for conducting a cellular assay, said disc comprising:
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a rotatable substrate;
a cap portion associated with said substrate; and
a fluidic circuit formed between said substrate and said cap portion, said fluidic circuit comprising;
a mixing chamber, said mixing chamber including an inlet port;
a purification chamber in fluid communication with said mixing chamber;
a filter means associated with said purification chamber;
an analysis chamber in fluid communication with said purification chamber, said analysis chamber including a capture zone; and
a vent port associated with said analysis chamber. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18)
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19. An optical bio disc for conducting a cellular assay, said optical bio-disc comprising:
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a rotatable substrate;
a microfluidic cassette attached to said substrate; and
a fluidic circuit formed within said microfluidic cassette. - View Dependent Claims (20, 21, 22)
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23. An optical disc and drive system for receiving a sample, the system comprising:
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a bio-disc comprising;
a substrate, a cap parallel to the substrate, a mixing chamber, purification chamber, and analysis chamber defined therebetween, said analysis chamber having capture zones; and
a capture layer over the substrate at the capture zones, such that a first capture zone has a first cell capture agent and a second capture zone has a second cell capture agent;
an optical disc drive comprising;
a light source for directing light to the disc at the capture zones, a detector for detecting light reflected from or transmitted through the disc at the capture zones and providing a signal; and
a processor for using the signal to count items in the sample bound to the capture molecules.
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24. A method of making an optical bio-disc for use in cellular assays, said method of making comprising the steps of:
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forming a rotatable optical disc substrate;
forming a cover disc with similar dimensions to said substrate;
forming a channel layer with cut out portions to form fluidic circuits;
said fluidic circuits including an inlet port, a mixing chamber, a purification chamber, an analysis chamber, and a vent port;
said analysis chamber including capture zones;
binding capture probes onto said capture zones;
attaching said channel layer to said substrate;
loading a filter means into said purification chamber;
attaching said cover disc to said channel layer to thereby form the optical bio-disc. - View Dependent Claims (25, 26, 27)
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Specification