Biocompatible poly-beta-1-4-N-acetylglucosamine

• US 20040087015A1
• Filed: 07/11/2003
• Published: 05/06/2004
• Est. Priority Date: 12/01/1993
• Status: Active Grant

• Current Assignee: Marine Polymer Technologies Incorporated
• Original Assignee: Marine Polymer Technologies Incorporated
• Inventors: Pariser, Ernst R., Finkielsztein, Sergio, Vournakis, John N., Helton, Mike
• Granted Patent: US 6,864,245 B2
• Time in Patent Office: Days
• US Class Current: 435/325
• CPC Class Codes: A61K 47/61 the organic macromolecular compound being a polysaccharide or a derivative thereof
• CPC Class Codes: A61K 47/69 the conjugate being characterised by physical or galenical forms, e.g. emulsion, particle, inclusion complex, stent or kit
• CPC Class Codes: A61K 9/205 Polysaccharides, e.g. alginate, gums; Cyclodextrin
• CPC Class Codes: A61L 15/28 Polysaccharides or their derivatives A61L15/225 takes precedence
• CPC Class Codes: A61L 31/042 Polysaccharides
• CPC Class Codes: B60R 2021/2633 with a plurality of inflation levels
• CPC Class Codes: B60R 2021/2725 using a fluid fuel gas generator
• CPC Class Codes: B60R 21/272 with means for increasing the pressure of the gas just before or during liberation, e.g. hybrid inflators
• CPC Class Codes: C06D 5/00 Generation of pressure gas, e.g. for blasting cartridges, starting cartridges, rockets explosive compositions containing an oxidizer, fuels for rocket engines intended for reaction with an oxidant other than air C06B
• CPC Class Codes: C08B 37/003 Chitin, i.e. 2-acetamido-2-deoxy-(beta-1,4)-D-glucan or N-acetyl-beta-1,4-D-glucosamine; Chitosan, i.e. deacetylated product of chitin or (beta-1,4)-D-glucosamine; Derivatives thereof
• CPC Class Codes: C08L 5/08 Chitin; Chondroitin sulfate; Hyaluronic acid; Derivatives thereof
• CPC Class Codes: C12N 1/125 Unicellular algae isolates
• CPC Class Codes: C12N 2533/70 Polysaccharides
• CPC Class Codes: C12N 5/0068 General culture methods using substrates for specific animal cell type C12N5/06
• CPC Class Codes: C12P 19/26 Preparation of nitrogen-containing carbohydrates
• CPC Class Codes: C12R 2001/89 Algae ; Processes using algae

Abstract

The present invention relates to a purified, easily produced poly-β-1→4-N-acetylglucosamine (p-GlcNAc) polysaccharide species. The p-GlcNAc of the invention is a polymer of high molecular weight whose constituent monosaccharide sugars are attached in β-1→4 conformation, and which is free of proteins, and substantially free of single amino acids, and other organic and inorganic contaminants. In addition, derivatives and reformulations of p-GlcNAc are described. The present invention further relates to methods for the purification of the p-GlcNAc of the invention from microalgae, preferably diatom, starting sources. Still further, the invention relates to methods for the derivatization and reformulation of the p-GlcNAc. Additionally, the present invention relates to the uses of pure p-GlcNAc, its derivatives, and/or its reformulations.

Claims

1. A method for immunoisolation of a cell comprising:

coating a cell/poly-β

-1→

4-N-acetylglucosamine species, said cell/poly-β

-1→

4-N-acetylglucosamine species comprising a poly-β

-1→

4-N-acetylglucosamine species comprising about 4,000 to about 150,000 N-acetylglucosamine monosaccharides covalently attached in a β

-1→

4 conformation, free of protein, substantially free of other organic contaminants, substantially free of inorganic contaminants, and having a molecular weight of about 800,000 daltons to about 30 million daltons within with at least one cell is encapsulated, with a coating having a polyelectrolyte charge opposite to the charge of the cell/poly-β

-1→

4-N-acetylglucosamine species, so that the cell within the cell/poly-β

-1→

4-N-acetylglucosamine species is immunoisolated.

2. The method of claim 1, wherein at least one acetylglucosamine monosaccharide of the poly-β

-1→

4-N-acetylglucosamine species as been deacetylated.