Modified ADAMTS4 molecules and method of use thereof
First Claim
1. An isolated, modified ADAMTS4 protein with improved stability compared to a naturally-occurring, full-length ADAMTS4 protein, said modified ADAMTS4 protein differing from the naturally-occurring, full-length ADAMTS4 protein by at least one amino acid.
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Abstract
The present invention relates to modified ADAMTS4 proteins having improved stability comparing to the corresponding native, unmodified proteins. The modified ADAMTS4 proteins can be expressed and isolated in large quantities, thus allowing further characterization of the proteins, such as crystallographic and enzyme kinetic studies. The purified, stable proteins would also facilitate the production of anti-ADAMTS antibodies and the development of inhibitors to ADAMTS enzymes.
17 Citations
20 Claims
- 1. An isolated, modified ADAMTS4 protein with improved stability compared to a naturally-occurring, full-length ADAMTS4 protein, said modified ADAMTS4 protein differing from the naturally-occurring, full-length ADAMTS4 protein by at least one amino acid.
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9. An isolated, modified ADAMTS4 protein with improved stability compared to an ADAMTS4 protein having an amino acid sequence recited in SEQ ID NO:
- 15, said modified ADAMTS4 protein comprising an amino acid sequence selected from the group consisting of SEQ ID NOS;
17, 19, 22, 24, 26, 27, 29, 31, 32, 40, and 46-53. - View Dependent Claims (11, 13, 15)
- 15, said modified ADAMTS4 protein comprising an amino acid sequence selected from the group consisting of SEQ ID NOS;
Specification