Novel luminescent metal chelates and methods for their detection
First Claim
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1. A chemical compound having one of the following formulas:
- MLP2(Bu), ML2P(Bu)where M is selected from the group consisting of ruthenium and osmium, P is a polydentate ligand of M selected from the group consisting of substituted bipyridines, non-substituted bipyridines, substituted phenathrolines, non-substituted phenanthrolines, and any combinations thereof, and L is a ligand of M wherein L is selected from the following;
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Abstract
The invention provides electrochemiluminescent metal chelates and methods for using these chelates in chemical and biological assays, particularly immunoassays and microarray assays.
148 Citations
32 Claims
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1. A chemical compound having one of the following formulas:
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MLP2(Bu), ML2P(Bu) where M is selected from the group consisting of ruthenium and osmium, P is a polydentate ligand of M selected from the group consisting of substituted bipyridines, non-substituted bipyridines, substituted phenathrolines, non-substituted phenanthrolines, and any combinations thereof, and L is a ligand of M wherein L is selected from the following;
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2. A chemical compound having the formula:
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ML3(Bu) where M is selected from the group consisting of ruthenium and osmium and L is a polydentate ligand of M wherein L is selected from the following;
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3. A method of detecting the presence of a substance of interest in a sample, comprising the steps of:
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a) contacting the sample and a chemical compound, the chemical compound having one of the following formulas; MLP2(Bu), ML2P(Bu) where M is selected from the group consisting of ruthenium and osmium, P is a polydentate ligand of M selected from the group consisting of substituted bipyridines, non-substituted bipyridines, substituted phenathrolines, non-substituted phenanthrolines, and any combinations thereof, and L is a ligand of M wherein L is selected from the following;
wherein R1, R2, R3, R4 and R5 could be H, alkyl, aryl, with any alkyl and aryl group having substituents selected from the moieties consisting of amino (NH2), thiol (SH), and carboxyl (COOH) groups, either individually or any combination thereof, including multiple or no substituents of each group, for binding with chemicals and biomolecules of interest; and
X is carbon or nitrogen and Bu is a substance that is attached the complex through P, L, or both;
b) inducing the chemical compound to electrochemiluminesce by exposing the chemical compound to electrochemical energy; and
c) detecting emitted electrochemiluminescence and thereby detecting the substance of interest. - View Dependent Claims (4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17)
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18. A method of detecting the presence of a substance of interest in a sample, comprising the steps of:
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a) contacting the sample and a chemical compound, the chemical compound having the formula; ML3(Bu) where M is selected from the group consisting of ruthenium and osmium and L is a polydentate ligand of M wherein L is selected from the following;
wherein R1, R2, R3, R4 and R5 could be H, alkyl, aryl, with any alkyl and aryl group having substituents selected from the moieties consisting of amino (NH2), thiol (SH), and carboxyl (COOH) groups, either individually or any combination thereof, including multiple substituents of each group wherein at least one of said substituents is present;
for binding with chemicals and biomolecules of interest;
X is selected from the group consisting of carbon and nitrogen; and
Bu is a substance that is attached to the complex through L;
b) inducing the chemical compound to electrochemiluminesce by exposing the chemical compound to electrochemical energy; and
c) detecting emitted electrochemiluminescence and thereby detecting the substance of interest. - View Dependent Claims (19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32)
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Specification