Methods and devices for producing a polymer at a location of a substrate

• US 20040166496A1
• Filed: 02/25/2003
• Published: 08/26/2004
• Est. Priority Date: 02/25/2003
• Status: Active Grant

• Current Assignee: Agilent Technologies, Inc.
• Original Assignee: Agilent Technologies, Inc.
• Inventors: Leproust, Eric M., Amorese, Douglas A.
• Granted Patent: US 7,252,938 B2
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B01J 19/0046 Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays synthesis methods per se C40B50/00
• CPC Class Codes: B01J 2219/00378 Piezoelectric or ink jet dispensers
• CPC Class Codes: B01J 2219/00576 fluorophore
• CPC Class Codes: B01J 2219/00691 using robots
• CPC Class Codes: B01J 2219/00693 Means for quality control
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01J 2219/00725 Peptides
• CPC Class Codes: C40B 40/06 Libraries containing nucleotides or polynucleotides, or derivatives thereof
• CPC Class Codes: C40B 40/10 Libraries containing peptides or polypeptides, or derivatives thereof
• CPC Class Codes: C40B 60/14 for creating libraries
• CPC Class Codes: Y10S 436/807 Apparatus included in process claim, e.g. physical support structures
• CPC Class Codes: Y10S 436/809 Multifield plates or multicontainer arrays

Abstract

Methods and devices for producing a polymer at a location of a substrate are provided. In the subject methods, a fluid droplet containing a first monomer labeled with a first detectable label is deposited from a fluid deposition device onto a location of a substrate surface having a second monomer labeled with a second detectable label. The first and second detectable labels are then detected to determine any misalignment between the fluid deposition device and the location of the substrate surface during deposition. Also provided are algorithms that perform the subject methods, as well as fluid deposition devices that include the subject algorithms. The subject invention also includes arrays produced according to the subject methods and kits that include the subject arrays.

Claims

1. A method for producing a polymer at a location of a substrate surface, said method comprising:

(a) depositing a fluid droplet containing a first monomer labeled with a first detectable label from a fluid deposition device onto said location of a substrate surface having a second monomer labeled with a second detectable label to produce a polymer at said location, wherein said first and second detectable labels are distinguishable from each other; and

(b) detecting said first and second detectable labels to determine any misalignment between said fluid deposition device and said location during said depositing.

2. The method according to claim 1, wherein said fluid deposition device is a pulse-jet fluid deposition device.

3. The method according to claim 1, further comprising adjusting said fluid deposition device and said substrate surface relative to each other if misalignment is detected.

4. The method according to claim 3, wherein said adjustment comprises adjusting at least one of a stage supporting said substrate and said fluid deposition device.

5. The method according to claim 4, wherein said stage is an XYZ stage.

6. The method according to claim 3, wherein said adjustment occurs prior to deposition of an additional monomer.

7. The method according to claim 1, wherein said first monomer is bonded to said second monomer upon said depositing.

8. The method according to claim 7, wherein said first monomer is bonded to a polymer that includes said second monomer upon said depositing, but is not bonded directly to said second monomer.

9. The method according to claim 1, wherein said polymer is a nucleic acid.

10. The method according to claim 1, wherein said polymer is a polypeptide.

11. The method according to claim 1, wherein said first and second detectable labels are fluorescent.

12. The method according to claim 1, wherein said detecting comprises optically scanning said substrate surface.

13. The method according to claim 1, wherein said method further comprises depositing said second monomer onto said location of said substrate with a fluid deposition device.

14. The method according to claim 13, wherein one or more additional monomers are deposited onto said location of said substrate prior to depositing said first monomer labeled with a first detectable label.

15. The method according to claim 1, wherein said method is a method of producing two different polymers at two different locations of said substrate.

16. The method according to claim 15, wherein said method is a method of producing a polymeric array.

17. The method according to claim 16, wherein said polymeric array is a nucleic acid array.

18. The method according to claim 16, wherein said polymeric array is a polypeptide array.

19. The method according to claim 1, wherein said first and second monomers are the same.

20. The method according to claim 1, wherein said first and second monomers are different.

21. An algorithm that determines any misalignment between a fluid deposition device and a location of a substrate surface during deposition of fluid from said fluid deposition device using detected signals according to claim 1, wherein said algorithm is present on a computer readable medium.

22. A fluid deposition device that deposits fluid onto a substrate surface, wherein said device includes a computer readable medium comprising an algorithm according to claim 21.

23. In a method of depositing fluid onto a substrate surface, the improvement comprising employing a fluid deposition device according to claim 22.

24. An array of polymers covalently bonded to a surface of a solid support, wherein said array includes at least one polymer covalently bonded to said surface that includes a first residue bonded to a first detectable label and a second residue bonded to a second detectable label, wherein said first and second detectable labels are distinguishable from each other.

25. A method of detecting the presence of an analyte in a sample, said method comprising:

(a) contacting a sample suspected of comprising said analyte with a polymeric array according to claim 24;

(b) detecting any binding complexes on the surface of the said array to determine the presence of said analyte in said sample using said binding complex data.

26. The method according to claim 25, wherein said method further comprises a data transmission step in which a result from a reading of the array is transmitted from a first location to a second location.

27. The method according to claim 26, wherein said reading is adjusted to correct for any misalignment.

28. The method according to claim 26, wherein said second location is a remote location.

29. A method comprising receiving data representing a result of a reading obtained by the method of claim 26.

30. A kit for use in an analyte detection method, said kit comprising:

(a) an array according to claim 24;

and

(b) instructions for using said array in an analyte detection method.