Biosensor and methods of use thereof

  • US 20040167383A1
  • Filed: 02/13/2004
  • Published: 08/26/2004
  • Est. Priority Date: 03/13/1998
  • Status: Active Grant
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First Claim
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1. A sampling system for monitoring the concentration of an analyte present in a biological system, said sampling system comprising:

  • (a) a reservoir containing an ionically conductive medium and an enzyme capable of reacting with the analyte to produce hydrogen peroxide;

    (b) sampling means for extracting the analyte from the biological system into the reservoir to obtain a sub-millimolar (sub-mM) concentration of the analyte in the reservoir which reacts with the enzyme to produce hydrogen peroxide, wherein said sampling means is in operative contact with the reservoir; and

    (c) a sensor element in operative contact with the reservoir, wherein said sensor element reacts electrochemically with the hydrogen peroxide produced in the reservoir to provide a detectable signal, and said sensor element comprises an electrode having a geometric surface area which ranges from about 0.1 to 3 cm2, a background current which ranges from about 2 to 60 nA or less when measured in a buffer solution at 0.6V, and a sensitivity which ranges from about 6 to 180 nA/μ

    M of hydrogen peroxide when measured in a buffer solution at 0.6V.

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