Biosensor, thin film electrode forming method, quantification apparatus, and quantification method
First Claim
1. A biosensor for quantifying a substrate included in a sample liquid comprising:
- a first insulating support and a second insulating support;
an electrode part comprising at least a working electrode and a counter electrode;
a specimen supply path for introducing the sample liquid to the electrode part; and
a reagent layer employed for quantifying the substrate included in the sample liquid, the electrode part, the specimen supply path, and the reagent layer existing between the first insulating support and the second insulating support, the specimen supply path being provided on the electrode part, and the reagent layer being provided on the electrode part in the specimen supply path, respectively, and the electrode part being dividedly formed by first slits provided on an electrical conductive layer which is formed on the whole or part of an internal surface of one or both of the first insulating support and the second insulating support.
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Accused Products
Abstract
A biosensor according to the present invention comprises a support 1, a conductive layer 2 composed of an electrical conductive material such as noble metal, for example gold, palladium or the like, and carbon, slits 3a and 3b parallel to the side of the support 1, slits 4a and 4b vertical to the side of the support 1, a working electrode 5, a counter electrode 6, a detecting electrode 7, a spacer 8 which covers the working electrode 5, the counter electrode 6 and the detecting electrode 7 on the support 1, a rectangular cutout part 9 forming a specimen supply path, an inlet 9a of the specimen supply path, a reagent layer 12 formed by applying a reagent including an enzyme or the like to the working electrode 5, the counter electrode 6 and the detecting electrode 7, which is exposed from the cutout part 9 of the spacer 8, and a cover 13 which covers the spacer 8, as shown in FIG. 1.
The so-constructed biosensor can be formed by a simple method, and a biosensor which is excellent in a measuring accuracy as well as a biosensor in which a reagent layer is placed uniformly on the electrodes regardless of a reagent liquid composition, and which has a uniform performance can be realized.
241 Citations
44 Claims
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1. A biosensor for quantifying a substrate included in a sample liquid comprising:
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a first insulating support and a second insulating support;
an electrode part comprising at least a working electrode and a counter electrode;
a specimen supply path for introducing the sample liquid to the electrode part; and
a reagent layer employed for quantifying the substrate included in the sample liquid, the electrode part, the specimen supply path, and the reagent layer existing between the first insulating support and the second insulating support, the specimen supply path being provided on the electrode part, and the reagent layer being provided on the electrode part in the specimen supply path, respectively, and the electrode part being dividedly formed by first slits provided on an electrical conductive layer which is formed on the whole or part of an internal surface of one or both of the first insulating support and the second insulating support. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 37, 38, 39, 40, 41, 42, 43, 44)
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24. A thin film electrode forming method for forming a thin film electrode on a surface of an insulating support including:
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a roughened surface forming step of roughening the surface of the insulating support by colliding an excited gas against the surface of the insulating support in a vacuum atmosphere; and
an electrical conductive layer forming step of forming the electrical conductive layer as a thin film electrode which is composed of a conductive substance on the roughened surface of the insulating support. - View Dependent Claims (25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36)
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Specification