Apparatus and method for imaging

• US 20040207846A1
• Filed: 05/07/2004
• Published: 10/21/2004
• Est. Priority Date: 07/11/2000
• Status: Active Grant

• Current Assignee: Maven Biotechnologies, LLC
• Original Assignee: David Ralin, Lothar U. Kempen, Robert A. Lieberman, William Rassman
• Inventors: Ralin, David, Rassman, William, Lieberman, Robert A., Kempen, Lothar U.
• Granted Patent: US 6,882,420 B2
• Time in Patent Office: Days
• US Class Current: 356/369
• CPC Class Codes: G01N 2021/212 Arrangement with total internal reflection
• CPC Class Codes: G01N 21/211 Ellipsometry optical thickness measurement G01B11/06
• CPC Class Codes: G01N 21/253 for batch operation, i.e. multisample apparatus analytical automats G01N35/00
• CPC Class Codes: G01N 21/552 Attenuated total reflection
• CPC Class Codes: G06F 9/264 Microinstruction selection based on results of processing
• CPC Class Codes: G06F 9/3846 using static prediction, e.g. branch taken strategy
• CPC Class Codes: G06F 9/3861 Recovery, e.g. branch miss-prediction, exception handling error detection or correction G06F11/00
• CPC Class Codes: Y10S 436/805 Optical property

Abstract

Apparatus for acquiring an image of a specimen comprising a cassette having an optical portion holding a specimen array on a TIR surface and being removably matable to a processing portion having a polarized light beam source and a processing polarization-sensitive portion to image the spatially distributed charges in polarization of the specimen array. In one form the array optical portion comprises a transparent slide having a bottom surface with first and second gratings located to direct polarized light to the TIR surface and to direct light reflected by that (TIR) surface to an imager, respectively. The apparatus may include a flow cell integral with the optical portion as well as means for selecting the direction and wavelength of the polarized light.

Claims

1-47. -47. (canceled)

48. A cassette for use with an imaging apparatus, said cassette comprising:

an optical element comprising a reflective surface to allow formation thereon of a specimen array, the optical element being configured to direct a polarized light beam from a light source to the reflective surface, such that a reflection of the polarized light beam at the reflective surface generates an evanescent field in the vicinity of the specimen array; and

a mount or frame coupled to the optical element and having a locating portion engageable with a mating locating element of a two-dimensional array detector adapted for providing an image based on spatially distributed polarization changes in the light beam.

49. The cassette of claim 48, wherein the optical element includes a prism.

50. The cassette of claim 49, wherein the optical element includes a transparent slide operably coupled to the prism.

51. The cassette of claim 48, wherein the cassette and the light source are configured for a single reflection of the light beam at the reflective surface.

52. The cassette of claim 48, wherein the mount or frame is operable to locate at least a selected portion of the specimen array in the evanescent field.

53. The cassette of claim 48, wherein the reflective surface comprises the upper surface of a transparent slide, the slide having a lower surface, the lower surface comprising at least one grating and the upper surface including an array of receptors thereon.

54. The cassette of claim 53, wherein the at least one grating comprises a first grating positioned to intercept the polarized light beam from the light source and a second grating positioned to intercept the light beam after it has reflected from the upper surface.

55. The cassette of claim 53, wherein the at least one grating comprises an electrically alterable grating.

56. The cassette of claim 53, wherein the at least one grating comprises a Bragg grating.

57. The cassette of claim 53, wherein the at least one grating comprises an electrically alterable hologram.

58. The cassette of claim 48, further comprising a flow cell secured to the reflective surface, the flow cell including an inlet port and an outlet port for flowing analyte across the specimen array.

59. A cassette for use with an imaging apparatus, said cassette comprising:

an optical element including a prism and a slide having a reflective surface to allow formation thereon of a specimen array, the optical element being configured to direct a polarized light beam from a light source to the reflective surface, such that a reflection of the polarized light beam at the reflective surface generates an evanescent field in the vicinity of the specimen array; and

a mount or frame coupled to the optical element and having a locating portion engageable with a mating locating element of a two-dimensional array detector adapted for providing an image based on spatially distributed polarization changes in the light beam, the mount or frame being operable to locate at least a selected portion of the specimen array in the evanescent field.

60. The cassette of claim 59, wherein the cassette and the light source are configured for a single reflection of the light beam at the reflective surface.

61. A cassette for use with an imaging apparatus, said cassette comprising:

an optical element comprising a reflective surface to allow formation thereon of a specimen array, said optical element being configured to direct a polarized light beam from a light source to the reflective surface, such that a reflection of the polarized light beam at the reflective surface generates an evanescent field in the vicinity of the specimen array; and

a mount or frame coupled to the optical element and having a locating portion engageable with a mating locating element of a processing assembly

62. The cassette of claim 61, wherein the processing assembly includes a two-dimensional array detector adapted for providing an image based on spatially distributed polarization changes in the light beam.

63. The cassette of claim 61, wherein the processing assembly includes the light source.

64. The cassette of claim 61, wherein the mount or frame is operable to locate at least a selected portion of the specimen array in the evanescent field.

65. The cassette of claim 61, wherein the reflective surface comprises the upper surface of a slide, the slide having a lower surface, the lower surface comprising at least one grating and the upper surface including an array of receptors thereon.

66. The cassette of claim 65, wherein the at least one grating comprises a first grating positioned to intercept the polarized light beam from the light source and a second grating positioned to intercept the light beam after it has reflected from the upper surface.

67. The cassette of claim 65, wherein the at least one grating comprises an electrically alterable grating.

68. The cassette of claim 65, wherein the at least one grating comprises a Bragg grating.

69. The cassette of claim 65, wherein the at least one grating comprises an electrically alterable hologram.

70. The cassette of claim 61, further comprising a flow cell secured to the reflective surface, the flow cell including an inlet port and an outlet port for flowing analyte across the specimen array.