Hyaluronic acid and chondroitin sulfate based hydrolyzed collagen type II and method of making same

• US 20040224384A1
• Filed: 06/17/2004
• Published: 11/11/2004
• Est. Priority Date: 11/13/2001
• Status: Active Grant

• Current Assignee: Biocell Technology LLC
• Original Assignee: Intellipi LLC
• Inventors: Ishaq, Suhail
• Granted Patent: US 7,091,180 B2
• Time in Patent Office: Days
• US Class Current: 435/68.1
• CPC Class Codes: A61K 2300/00 Mixtures or combinations of active ingredients, wherein at least one active ingredient is fully defined in groups A61K31/00 - A61K41/00
• CPC Class Codes: A61K 35/32 Bones; Osteocytes; Osteoblasts; Tendons; Tenocytes; Teeth; Odontoblasts; Cartilage; Chondrocytes; Synovial membrane
• CPC Class Codes: A61K 35/60 Fish, e.g. seahorses; Fish eggs
• CPC Class Codes: A61K 38/39 Connective tissue peptides, e.g. collagen, elastin, laminin, fibronectin, vitronectin, cold insoluble globulin [CIG]
• CPC Class Codes: A61P 19/04 for non-specific disorders of the connective tissue
• CPC Class Codes: C12P 19/26 Preparation of nitrogen-containing carbohydrates

Abstract

Hydrolyzed collagen type II powder compositions for inducing cartilage formation in an individual, method of preparing the compositions and use of the compositions in treating connective tissue disorder, replenishing skin viscoelasticity. The compositions are administered through an orally ingestible delivery medium for absorption into the gastrointestinal tract. The compositions are administered through a topical delivery medium for absorption into a dermis of the individual.

Claims

1. A method for preparing chicken sternal cartilage-derived material comprising hydrolyzed collagen type II, comprising the following steps:

(a) cutting fresh chicken sternal cartilage to within not less than about 2 mm of the bone;

(b) suspending said cartilage in an aqueous solution;

(c) treating said cartilage with a proteolytic enzyme to form a hydrolysate, said proteolytic enzyme being capable of hydrolyzing collagen type II to fragments having an average molecular weight of between about 50 to about 10,000 daltons;

(d) sterilizing said hydrolysate;

(e) filtering said hydrolysate;

(f) concentrating said hydrolysate;

(g) drying said hydrolysate to form a powder enriched in collagen type II powder; and

(h) isolating said powder enriched in collagen type II.

2. The method of claim 1, further comprising the step of freezing said cartilage after said cutting step.

3. The method of claim 1, wherein said aqueous solution is water.

4. The method of claim 1, wherein said enzyme is selected from the group consisting of papain, ficin and bromelain.

5. The method of claim 1, wherein said sterilizing step comprises heating said hydrolysate at about 95°

C.-105°

C. for about 30 minutes.

6. The method of claim 1, wherein said drying step comprises spray drying.

7. The method of claim 1, wherein step (b) is performed at a pH between 4 and 8.

8. The method of claim 1, wherein step (c) is performed at a pH between 4 and 8.

9. The method of claim 1, wherein said material comprises depolymerized chondroitin sulfate.

10. The method of claim 9, wherein said depolymerized chondroitin sulfate is at least 20% of said material.

11. The method of claim 1, wherein said powder comprises hyaluronic acid.

12. The method of claim 11, wherein said hyaluronic acid is at least 10% of said material.

13. Hydrolyzed collagen type II for inducing cartilage formation in an individual, said hydrolyzed collagen being derived from chicken sternal cartilage, said hydrolyzed collagen having an average molecular weight of between about 50 and about 10,000 daltons.

14. The hydrolyzed collagen type II of claim 13, wherein said hydrolyzed collagen is formed into an orally ingestible delivery medium.

15. The hydrolyzed collagen type II of claim 14, wherein said orally ingestible delivery medium is chosen from the group consisting of powder, tablet, aqueous suspension, emulsion, capsule, elixir and combinations thereof.

16. The hydrolyzed collagen type II of claim 13, wherein said hydrolyzed collagen is formed into a topical delivery medium.

17. The hydrolyzed collagen type II of claim 16, wherein said topical delivery medium is a topical gel absorbable into a dermis.

18. The hydrolyzed collagen type II of claim 13, wherein said hydrolyzed collagen is derived from a mammalian cartilage.

19. The hydrolyzed collagen type II of claim 13, wherein said hydrolyzed collagen is derived from a shark fin.

20. The hydrolyzed collagen type II of claim 13, wherein said hydrolyzed collagen comprises depolymerized chondroitin sulfate.

21. The hydrolyzed collagen type II of claim 20, wherein said depolymerized chondroitin sulfate is at least 20% of said hydrolyzed collagen.

22. The hydrolyzed collagen type II of claim 13, wherein said hydrolyzed collagen comprises hyaluronic acid.

23. The hydrolyzed collagen type II of claim 22, wherein said hyaluronic acid is at least 10% of said hydrolyzed collagen.

24. A method for treating connective tissue disorder comprising orally administering an effective daily amount of chicken sternal cartilage-derived material comprising hydrolyzed collagen type II having an average molecular weight of between about 50 and about 10,000 daltons.

25. The method of claim 24, wherein said hydrolyzed collagen comprises depolymerized chondroitin sulfate.

26. The method fo claim 25, wherein said depolymerized chondroitin sulfate is at least 20% of said hydrolyzed collagen.

27. The method of claim 24, wherein said hydrolyzed collagen comprises hyaluronic acid.

28. The method of claim 27, wherein said hyaluronic acid is at least 10% of said hydrolyzed collagen.

29. A method of replenishing hyaluronic acid and chondroitin sulfate comprising topically administering a selectable amount of chicken sternal cartilage-derived material to a dermis, said material comprising hydrolyzed collagen type II having an average molecular weight of between about 50 and about 10,000 daltons.

30. The method of claim 29, wherein said hydrolyzed collagen comprises depolymerized chondroitin sulfate.

31. The method fo claim 30, wherein said depolymerized chondroitin sulfate is at least 20% of said hydrolyzed collagen.

32. The method of claim 29, wherein said hydrolyzed collagen comprises hyaluronic acid.

33. The method of claim 32, wherein said hyaluronic acid is at least 10% of said hydrolyzed collagen.