Amperometric biosensor in thick film technology

• US 20040241746A1
• Filed: 02/09/2004
• Published: 12/02/2004
• Est. Priority Date: 08/09/2001
• Status: Active Grant

• Current Assignee: Alexander Adlassnig, Julius Schuster
• Original Assignee: Alexander Adlassnig, Julius Schuster
• Inventors: Adlassnig, Alexander, Schuster, Julius
• Granted Patent: US 7,442,289 B2
• Time in Patent Office: Days
• US Class Current: 435/7.1
• CPC Class Codes: C12Q 1/005 involving specific analytes or enzymes including groups of enzymes, e.g. oxydases; C12Q1/004 takes precedence
• CPC Class Codes: C12Q 1/006 for glucose

Abstract

Described is an amperometric biosensor in thick film technology for the detection an/or determination of substances undergoing enzyme catalyzed reactions, especially glucose or lactate, comprising an inert carrier material, at least one transducer layer exhibiting a specific electrical conductivity χ>10⁴ Ω⁻¹ cm⁻¹, and at least one bioactive layer with diffusion barrier function containing at least one enzyme specifically reacting with said substance to be measured, said bioactive layer exhibiting a specific electrical conductivity χ<1 Ω⁻¹ cm⁻¹ and said bioactive layer further effectively hinders other electroactive substances to reach the transducer layer within the time of measurement.

Claims

1-5. -5. (Canceled).

6. A thick film amperometric biosensor capable of detection and/or determination of a substance undergoing an enzyme-catalyzed reaction comprising:

an inert carrier material;

at least one transducer layer exhibiting a specific electrical conductivity χ

>

10⁴ Ω

<sup>−

1</sup>cm<sup>−

1</sup>, during use of the biosensor; and

at least one bioactive layer adapted to function as a diffusion barrier during use of the biosensor, the bioactive layer further defined as;

comprising at least one enzyme capable of specifically reacting with the substance to be detected or determined during use of the biosensor;

exhibiting a specific electrical conductivity of χ

<

1 Ω

<sup>−

1</sup>cm<sup>−

1</sup> during use of the biosensor; and

capable of effectively hindering electroactive substances, other than any produced by the substance undergoing the enzyme-catalyzed reaction, from reaching the transducer layer during use of the biosensor.

7. The biosensor of claim 6, further defined as adapted for the detection and/or determination of glucose or lactate undergoing an enzyme-catalyzed reaction.

8. The biosensor of claim 6, wherein the at least one bioactive layer comprises two or more zones.

9. The biosensor of claim 8, wherein at least one of the at least two or more zones is a zone adjacent to the electrically conducting layer that is depleted of bioactive substances.

10. A process for producing a thick film amperometric biosensor capable of detection and/or determination of a substance undergoing an enzyme-catalyzed reaction comprising:

providing an inert carrier material;

depositing at least one electrically conducting layer on the inert carrier material; and

depositing at least one bioactive layer capable of functioning as a diffusion barrier during use of the biosensor onto the at least one electrically conducting layer by screen printing using a transducer-free printing ink comprising an enzyme and a biocompatible polymer, this depositing being accomplished without the use of UV-radiation.

11. The process of claim 10, wherein the at least one bioactive layer is produced from a printing ink that comprises at least one bioactive substance but is free of components that could act as transducers during use of the biosensor.

12. A printing ink comprising an enzyme, wherein the ink is screen printable without the use of UV-radiation and capable of, in an amperometric biosensor capable of detection and/or determination of a substance undergoing an enzyme-catalyzed reaction, forming at least one bioactive layer, adapted to:

function as a diffusion barrier during use of the biosensor;

have a specific electrical conductivity of χ

<

1 Ω

<sup>−

1</sup>cm<sup>−

1</sup> during use of the biosensor; and

effectively hinder electroactive substances other than any produced by the substance undergoing the enzyme-catalyzed reaction from reaching a transducer layer during use of the biosensor.

13. The printing ink of claim 12, further defined as free of components that could act as transducers during use of the biosensor.

14. A method of detection and/or determination of a substance undergoing an enzyme-catalyzed reaction comprising:

obtaining a thick film amperometric biosensor capable of detection and/or determination of a substance undergoing an enzyme-catalyzed reaction comprising;

(a) an inert carrier material;

(b) at least one transducer layer exhibiting a specific electrical conductivity χ

>

10⁴ Ω

<sup>−

1</sup>cm<sup>−

1</sup>, during use of the biosensor; and

(c) at least one bioactive layer adapted to function as a diffusion barrier during use during use of the biosensor, the bioactive layer further defined as;

(i) comprising at least one enzyme capable of specifically reacting with the substance to be detected or determined during use of the biosensor;

(ii) exhibiting a specific electrical conductivity of χ

<

1 Ω

<sup>−

1</sup>cm<sup>−

1</sup> during use of the biosensor; and

(iii) capable of effectively hindering electroactive substances, other than any produced by the substance undergoing the enzyme-catalyzed reaction, from reaching the transducer layer during use of the biosensor;

obtaining a sample; and

using the biosensor to detect and/or determine a substance in the sample.