Binding acceleration techniques for the detection of analytes

• US 20050003399A1
• Filed: 04/12/2004
• Published: 01/06/2005
• Est. Priority Date: 06/23/1998
• Status: Active Grant

• Current Assignee: Clinical Micro Sensors Inc. (Roche Holding AG)
• Original Assignee: Osmetech Technology Inc. (Roche Holding AG)
• Inventors: O'Connor, Stephen D., Meade, Thomas J., Terbrueggen, Robert H., Vielmetter, Jost G., Creager, Stephen E., Blackburn, Gary, Irvine, Bruce D., Fraser, Scott, Welch, Thomas W.
• Granted Patent: US 7,655,129 B2
• Time in Patent Office: Days
• US Class Current: 435/6
• CPC Class Codes: B82Y 15/00 Nanotechnology for interacting, sensing or actuating, e.g. quantum dots as markers in protein assays or molecular motors
• CPC Class Codes: B82Y 30/00 Nanotechnology for materials or surface science, e.g. nanocomposites
• CPC Class Codes: C12Q 1/6825 Nucleic acid detection involving sensors
• CPC Class Codes: C12Q 2565/629 being a microfluidic device

Abstract

The invention relates to compositions and methods useful in the acceleration of binding of target analytes to capture ligands on surfaces. Detection proceeds through the use of an electron transfer moiety (ETM) that is associated with the target analyte, either directly or indirectly, to allow electronic detection of the ETM.

Claims

1. A method of detecting a target analyte in a sample comprising:

a) adding said sample to a detection chamber comprising array of detection electrodes, each comprising a covalently attached capture ligand;

b) mixing said sample such that said target analyte binds to said capture ligand to form an assay complex, wherein said assay complex further comprises at least one electron transfer moiety (ETM); and

c) detecting the presence of said ETM using said detection electrodes.

2. A method according to claim 1 wherein said capture ligand comprises a nucleic acid.

3. A method according to claim 1 wherein each of said detection electrodes further comprise a self assembled monolayer.

4. A method according to claim 1 wherein said mixing is accomplished by applying an AC/DC pulse.

5. A method according to claim 1 wherein said mixing is accomplished through the use of mixing particles.

6. A method according to claim 5 wherein said mixing particles comprise microparticulate matter.

7. A method according to claim 1 wherein said mixing is accomplished through the use of an electrophoretic electrode.

8. A method according to claim 1 wherein each of said detection electrodes is “

sunken”

or “

recessed”

with respect to the chamber, such that the flow of said sample past each of said detection electrodes causes said mixing.