Novel metarhizium genus microorganism and the method for controlling the soil pests using the same

• US 20050019309A1
• Filed: 11/02/2002
• Published: 01/27/2005
• Est. Priority Date: 11/02/2001
• Status: Active Grant

• Current Assignee: Korea Research Institute of Bioscience & Biotechnology
• Original Assignee: Korea Research Institute of Bioscience & Biotechnology
• Inventors: Park, Ho Yong, Kim, Ki-Duk, Son, Kwang Hee, Suh, Eun Young, Shin, Dong Ha
• Granted Patent: US 7,416,880 B2
• Time in Patent Office: Days
• US Class Current: 424/93.500
• CPC Class Codes: A01N 2300/00 Combinations or mixtures of active ingredients covered by classes A01N27/00 - A01N65/48 with other active or formulation relevant ingredients, e.g. specific carrier materials or surfactants, covered by classes A01N25/00 - A01N65/48
• CPC Class Codes: A01N 25/14 wettable
• CPC Class Codes: A01N 25/30 characterised by the surfactants
• CPC Class Codes: A01N 63/30 Microbial fungi; Substances produced thereby or obtained therefrom
• CPC Class Codes: C12N 1/14 Fungi culture of mushrooms A01G18/00; as new plants A01H15/00; Culture media therefor
• CPC Class Codes: C12N 1/145 Fungal isolates
• CPC Class Codes: C12R 2001/645 Fungi ; Processes using fungi

Abstract

The present invention relates to a novel fungus of Metarhizium genus and a method for controlling soil pests using the same, more particularly to a novel Metarhizium anisopliae HY-2 and a microbial insecticide comprising the same and a method for controlling soil pests using the same. The Metarhizium anisopliae HY-2 can effectively be used to control the soil pests such as Scarabaeidae.

Claims

1. A Metarhizium anisopliae fungus designated HY-2 having insecticidal activity to soil pests, deposited under Gene Bank of Korea Research Institute of Bioscience and Biotechnology under Accession No:

KCTC 01 56BP.

3. A medium for mass-production of the fungus of claim 1 containing carbon sources, nitrogen sources and inorganic elements.

4. The medium as set forth in claim 3, wherein the carbon source is selected from a group consisting of glucose, cornstarch, saccharose, molasses, wheat bran and rice bran, the nitrogen source is selected from a group consisting of yeast extract, soybean flour, corn-immersion, malt extract and peptone, and the inorganic element is selected from a group consisting of potassium chloride, magnesium sulfonate, ferroic sulfate and sodium nitrate.

5. A microbial insecticide for controlling soil pests containing the fungus of claim 1 as an effective ingredient.

6. The microbial insecticide of claim 5, wherein the microbial insecticide is prepared in the forms of wettable powders, granules or capsules.

7. The microbial insecticide of claim 6, wherein the wettable powders are prepared by:

pulverizing dried solid media inoculated with Metarhizium anisopliae HY-2, thereby generating a pulverized powder; and

adding surfactants and diluents to the pulverized powders; and

pulverizing there pulverized powder.

8. The microbial insecticide as set forth of claim 6, wherein the granules are prepared by the following steps:

pulverizing dried solid media inoculated with Metarhizium anisopliae HY-2, thereby generating a pulverized powder;

adding surfactants, adjuvant and diluents to the pulverized powder;

kneading thereef surfactants, adjuvant, diluents and pulverized powder with water, thereby generating a dough; and

granulating the dough; and

drying the dough.

9. The microbial insecticide of claim 7, wherein the surfactant is polycarboxylate, sodium lingo sulfate, sodium dialkyl sulfosuccinate, sodium alkyl sulfonate, polyoxyethylene alkyl phenyl ether, sodium tripolyphosphate, polyoxyethylene alkyl aryl phosphoric ester, polyoxyethylene alkyl aryl ether, polyoxyethylene alkyl arylpolymer, polyoxyalkylon alkyl phenyl ether, polyoxyethylene nonyl phenyl ether, sodium sulfonate or naphthalene formaldehyde.

10. The insecticide as set forth in claim 6, wherein the granules are prepared with the following weight ratios:

Metarhizium anisopliae HY-2 spores 10-60 weight parts, surfactants 2-16 weight parts, nutrients 5-20 weight parts, disintegrators 10-30 weight parts.

11. The microbial insecticide of claim 10, wherein the granules additionally contained one or more components selected from a group consisting of inactive carriers, preservatives, wetting agents, supply accelerants, attractants, encapsulating agents, binders, emulsifiers, dyes, UV protectors, buffers and fluids.

12. The microbial insecticide of claim 8, wherein the surfactant is s polycarboxylate, sodium lingo sulfate, sodium dialkyl sulfosuccinate, sodium alkyl sulfonate, polyoxyethylene alkyl phenyl ether, sodium tripolyphosphate, polyoxyethylene alkyl aryl phosphoric ester, polyoxyethylene alkyl aryl ether, polyoxyethylene alkyl arylpolymer, polyoxyalkylon alkyl phenyl ether, polyoxyethylene nonyl phenyl ether, sodium sulfonate or naphthalene formaldehyde.

2. (canceled)