High speed, consecutive batch or continuous, low effluent process for the production of ethanol from molasses, starches, or sugars
First Claim
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1. A method of developing an improved strain of Saccharomyces cerevisae which comprises a) a selecting a highly flocculent strain of that species, b) running a continuous single stage CSTR for a period of weeks, c) selecting, based on floc stability, a ‘
- best’
pellet, d) using this pellet as a starting inoculation for running a second CSTR, e) repeating steps b), c) and d) until an obtained stable well shaped floc cell mass is capable of maintaining consistent performance over a period of time, f) improving the osmo-tolerance of the stable floc mutant obtained from step d) by growing a small pellet thereof in a high-salt medium, g) taking a single pellet from the resulting medium and growing it again in fresh medium, h) repeating steps f) and g) a number of times until a new, highly flocculent, stable, osmo-tolerant mutant strain of Saccharomyces cerevisae is obtained.
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Abstract
A flocculent Saccharomyces cerevisae strain (BPSC-15) produces higher yields of ethanol in fermentations of fermentable sugar or fermentable starch/enzyme.
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7 Claims
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1. A method of developing an improved strain of Saccharomyces cerevisae which comprises a) a selecting a highly flocculent strain of that species, b) running a continuous single stage CSTR for a period of weeks, c) selecting, based on floc stability, a ‘
- best’
pellet, d) using this pellet as a starting inoculation for running a second CSTR, e) repeating steps b), c) and d) until an obtained stable well shaped floc cell mass is capable of maintaining consistent performance over a period of time, f) improving the osmo-tolerance of the stable floc mutant obtained from step d) by growing a small pellet thereof in a high-salt medium, g) taking a single pellet from the resulting medium and growing it again in fresh medium, h) repeating steps f) and g) a number of times until a new, highly flocculent, stable, osmo-tolerant mutant strain of Saccharomyces cerevisae is obtained. - View Dependent Claims (2, 3, 4, 5, 6)
- best’
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7. A biologically pure culture of a Saccharomyces cerevisae strain which i) has the capability to maintain a totally floc mode characterized by yeast pellets of from 0.1 to 5 mm in diameter in a fermentation medium, ii) shows a yeast free cell (single or budding double cells) of less than 0.5 g/L with a yeast floc density as high as 100 g/L or higher, and iii) has a limiting osmo-tolerance for ethanol productivity of about 5.0 os/kg.
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