Compositions and methods for priming monocytic dendritic cells and t cells for th-1response
First Claim
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1. A method for producing a mature dendritic cell population, comprising:
- providing immature dendritic cells; and
contacting the immature dendritic cells with an effective amount of BCG and Interferon gamma (IFNγ
) under culture conditions suitable for maturation of the immature dendritic cells to form a mature dendritic cell population;
wherein the mature dendritic cell population produces an increased ratio of Interleukin 12 to Interleukin 10 than an immature dendritic cell population not contacted with BCG and IFNγ
during maturation.
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Abstract
The present invention provides compositions and methods for inducing maturation of immature dendritic cells (DC) and for priming those cells for inducing a type 1 immune response. The present invention also provides dendritic cell populations useful for activating and for preparing T cells polarized towards production of type 1 cytokines and/or a type 1 response. Similarly, activated, polarized T cell populations, and methods of making the same are provided.
163 Citations
45 Claims
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1. A method for producing a mature dendritic cell population, comprising:
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providing immature dendritic cells; and
contacting the immature dendritic cells with an effective amount of BCG and Interferon gamma (IFNγ
) under culture conditions suitable for maturation of the immature dendritic cells to form a mature dendritic cell population;
wherein the mature dendritic cell population produces an increased ratio of Interleukin 12 to Interleukin 10 than an immature dendritic cell population not contacted with BCG and IFNγ
during maturation. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8)
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9. A method for producing a mature dendritic cell population, comprising:
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providing immature dendritic cells; and
contacting the immature dendritic cells with an effective amount of BCG and Interferon gamma (IFNγ
) under culture conditions suitable for maturation of the immature dendritic cells to form a mature dendritic cell population;
wherein the mature dendritic cell population produces a type 1 immune response. - View Dependent Claims (10, 11, 12, 13, 14, 15, 16)
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17. A composition for activating T cells, comprising:
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a dendritic cell populations matured with an effective concentration of BCG and IFNγ
under suitable conditions for maturation; and
a predetermined antigen;
wherein the dendritic cell population produces an increased ratio of Interleukin 12 (IL-12) to Interleukin 10 (IL-10) than a mature dendritic cell population contacted with BCG without IFNγ
during maturation. - View Dependent Claims (18, 19)
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20. An isolated, immature dendritic cell population, comprising:
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isolated immature monocytic dendritic cells, and an effective concentration of BCG and IFNγ
to induce maturation of the immature dendritic cells;
wherein the resulting mature dendritic cells produce more Interleukin 12 (IL-12) to Interleukin 10 (IL-10). - View Dependent Claims (21, 22, 23, 24)
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25. A method for producing T cells, comprising:
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providing immature dendritic cells;
contacting the immature dendritic cells with a predetermined antigen;
contacting the immature dendritic cells with an effective concentration of BCG and IFNγ
under culture conditions suitable for maturation of the immature dendritic cells to form mature dendritic cells; and
contacting the mature dendritic cells with naï
ve T cells to form an activated T cells producing IFNγ
. - View Dependent Claims (26, 27, 28, 29, 30, 31)
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32. Isolated mature dendritic cells producing more Interleukin 12 (IL-12) to Interleukin 10 (IL-10) prepared by maturation of immature dendritic cells with a composition comprising effective concentrations of BCG and IFNγ
- under conditions suitable for the maturation of the dendritic cells.
- View Dependent Claims (33)
- 34. Isolated mature dendritic cells loaded with a predetermined antigen, the dendritic cells producing more Interleukin 12 (IL-12) than Interleukin 10 (IL-10).
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36. A method for producing a type 1 immune response in an animal, comprising:
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providing immature dendritic cells;
contacting the immature dendritic cells with effective amounts of BCG and Interferon gamma (IFNγ
) and a predetermined antigen under culture conditions suitable for maturation of the immature dendritic cells to form mature dendritic cells;
contacting the mature dendritic cells with naï
ve T cells to form activated T cells producing more Interleukin 12 (IL-12) to Interleukin 10 (IL-10); and
administering the activated T cells to the animal. - View Dependent Claims (37, 38, 39, 40, 41, 42, 43, 44, 45)
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Specification