Determination of disease state using Raman Spectroscopy of tissue
First Claim
1. A method of determining a disease state in an individual, comprising:
- a. illuminating a portion of the tissue of the individual with excitation light;
b. detecting light emitted from the tissue by Raman scattering of a chemical within the tissue;
c. determining the disease state from the detected light and a model relating Raman scattering and disease state.
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Accused Products
Abstract
A method of determining disease state in an individual. A portion of the tissue of the individual is illuminated with excitation light, then light emitted by the tissue due to Raman scattering of a chemical with the tissue responsive to the excitation light is detected. The detected light can be combined with a model relating Raman emission with disease state to determine a disease state of the individual. The invention can comprise single wavelength excitation light, scanning of excitation light (illuminating the tissue at a plurality of wavelengths), detection at a single wavelength, scanning of detection wavelengths (detecting emitted light at a plurality of wavelengths), and combinations thereof. The invention also can comprise correction techniques that reduce determination errors due to detection of light other than that from Raman emission of a chemical in the tissue. For example, the reflectance of the tissue can lead to errors if appropriate correction is not employed. The invention can also comprise a variety of models relating Raman emission to disease state, including a variety of methods for generating such models. Other biologic information can be used in combination with the Raman spectral properties to aid in the determination of disease state. The invention also comprises apparatuses suitable for carrying out the method, including appropriate light sources, detectors, and models (for example, implemented on computers) used to relate detected Raman emission and disease state.
63 Citations
42 Claims
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1. A method of determining a disease state in an individual, comprising:
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a. illuminating a portion of the tissue of the individual with excitation light;
b. detecting light emitted from the tissue by Raman scattering of a chemical within the tissue;
c. determining the disease state from the detected light and a model relating Raman scattering and disease state. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 41)
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19. A method of determining a disease state in an individual, comprising:
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a. establishing an interface between a optical system and a portion of the skin of the individual;
b. determining a relationship of illumination light at an excitation wavelength and the response of the skin at a detection wavelength for each of a plurality of pairings of excitation wavelength and detection wavelength;
c. determining a tissue reflectance property of the skin at each of the illumination wavelengths and at each of the detection wavelengths;
d. determining a measure of the intrinsic Raman emission of the skin from the relationships of illumination light and detected light and the tissue reflectance properties;
e. determining the disease state of the individual from the intrinsic Raman emission using a model relating intrinsic Raman emission and disease state. - View Dependent Claims (20, 21, 22)
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23. A method of determining a model relating Raman emission and disease state, comprising:
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a. for each of a plurality of subjects;
i. determining a Raman emission property of a portion of the tissue of the subject;
ii. determining a disease state of the subject;
b. applying a multivariate method to the plurality of Raman emission property determinations and associated disease state determinations to generate a model relating Raman emission property to disease state. - View Dependent Claims (24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 42)
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34. An apparatus for the determination of disease state in an individual, comprising:
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a. an illumination subsystem;
b. a detection subsystem;
c. an analysis subsystem, comprising a model relating Raman emission property of the skin of an individual to disease state. - View Dependent Claims (35)
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36. A method of determining disease state in an individual, comprising:
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a. determining a Raman emission property of a portion of the skin of the individual;
b. using a multivariate method to determine a disease state of the individual from the Raman emission property. - View Dependent Claims (37, 38, 39, 40)
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Specification