Methods for producing and using in vivo pseudotyped retroviruses using envelope glycoproteins from lymphocytic choriomeningitis virus (LCMV)
First Claim
Patent Images
1. A method for transducing a nervous system cell with a transgene, said method comprising contacting the cell with a pseudotyped retrovirus virion comprising a Lymphocytic Choriomeningitis Virus strain WE-54 envelope glycoprotein and the transgene.
2 Assignments
0 Petitions
Accused Products
Abstract
The present invention provides novel pseudotyped retroviral vectors that can transduce human and other cells. Vectors are provided that are packaged efficiently in packaging cells and cell lines to generate high titer recombinant virus stocks expressing novel envelope glycoproteins. The present invention further relates to compositions for gene therapy.
27 Citations
15 Claims
- 1. A method for transducing a nervous system cell with a transgene, said method comprising contacting the cell with a pseudotyped retrovirus virion comprising a Lymphocytic Choriomeningitis Virus strain WE-54 envelope glycoprotein and the transgene.
- 7. A method for transducing a nervous system cell with a transgene, said method comprising contacting the neural cell with a vector comprising (a) a nucleic acid encoding an envelope glycoprotein from Lymphocytic Choriomeningitis Virus strain WE-54, and (b) the transgene.
- 11. A method for treating a mammal diagnosed with a neurogenetic disorder, the method comprising administering to said mammal a pseudotyped retrovirus virion comprising (a) a Lymphocytic Choriomeningitis Virus strain WE-54 envelope glycoprotein and (b) a transgene.
Specification