Biosensor

• US 20050158850A1
• Filed: 12/27/2004
• Published: 07/21/2005
• Est. Priority Date: 12/25/2003
• Status: Active Grant

• Current Assignee: Fujifilm Corporation (Fujifilm Holdings Corporation)
• Original Assignee: Fuji Photo Film Co Limited (Fujifilm Holdings Corporation)
• Inventors: Kubo, Toshiaki, Ezoe, Toshihide
• Granted Patent: US 7,501,289 B2
• Time in Patent Office: Days
• US Class Current: 435/287.200
• CPC Class Codes: G01N 21/553 and using surface plasmons fluorescence excitation G01N21/648; enhanced Raman G01N21/658
• CPC Class Codes: Y10S 436/805 Optical property

Abstract

It is an object of the present invention to provide a biosensor, which is not significantly affected by the baseline fluctuation and suppresses nonspecific adsorption. The present invention provides a biosensor, which comprises a metal surface or metal film coated with a hydrophobic polymer, and has two or more types of different surfaces in a region coated with a hydrophobic polymer.

Claims

1. A biosensor, which comprises a metal surface or metal film coated with a hydrophobic polymer, and has two or more types of different surfaces in a region coated with a hydrophobic polymer.

2. The biosensor according to claim 1, wherein at least a measurement unit to which a physiologically active substance or a substance interacting therewith binds and a measurement unit that does not have a physiologically active substance or a substance interacting therewith exit on a single plane.

3. The biosensor according to claim 1, wherein the metal surface or metal film consists of a free electron metal selected from the group consisting of gold, silver, copper, platinum, and aluminum.

4. The biosensor according to claim 1, wherein the thickness of the metal film is between 1 angstrom and 5,000 angstroms.

5. The biosensor according to claim 1, wherein the coating thickness of the hydrophobic polymer is between 1 angstrom and 5,000 angstroms.

6. The biosensor according to claim 1, which is used in non-electrochemical detection.

7. The biosensor according to claim 1, which is used in surface plasmon resonance analysis.

8. The biosensor according to claim 1, which is formed in a measurement chip that is used for a surface plasmon resonance measurement device comprising a dielectric block, a metal film formed on one side of the dielectric block, a light source for generating a light beam, an optical system for allowing said light beam to enter said dielectric block so that total reflection conditions can be obtained at the interface between said dielectric block and said metal film and so that various incidence angles can be included, and a light-detecting means for detecting the state of surface plasmon resonance by measuring the intensity of the light beam totally reflected at said interface,

wherein said measurement chip is basically composed of said dielectric block and said metal film, wherein said dielectric block is formed as a block including all of an incidence face and an exit face for said light beam and a face on which said metal film is formed, and wherein said metal film is unified with this dielectric block.

9. A method for producing the biosensor according to claim 1, which comprises steps of coating a substrate with a hydrophobic polymer, and producing two or more types of surfaces on a single plane without allowing a solid to come into contact with a detection region.

10. The method for producing the biosensor according to claim 9, wherein at least two types of surfaces are produced on a single plane using a diaphragm(s).

11. A method for immobilizing a physiologically active substance on a biosensor, which comprises a step of allowing a physiologically active substance to come into contact with the biosensor according to claim 1, so as to allow said physiologically active substance to bind to the surface of said biosensor via a covalent bond.

12. A method for detecting or measuring a substance interacting with a physiologically active substance, which comprises a step of allowing a test substance to come into contact with the biosensor according to claim 1 to the surface of which the physiologically active substance binds via a covalent bond.

13. The method according to claim 12, wherein the substance interacting with the physiologically active substance is detected or measured by a non-electrochemical method.

14. The method according to claim 12, wherein the substance interacting with the physiologically active substance is detected or measured by surface plasmon resonance analysis.

15. A biosensor comprising a substrate which is coated with a hydrophobic polymer, to the surface of which a hydrophilic compound binds.

16. The biosensor according to claim 15, wherein the molecular weight of the hydrophilic compound is between 50 and 20,000.

17. The biosensor according to claim 15, wherein one group binding to one molecule of the physiologically active substance exists in one molecule of the hydrophilic compound.

18. The biosensor according to claim 15, wherein 2 to 1,000 groups binding to a physiologically active substance exist in one molecule of the hydrophilic compound.

19. The biosensor according to claim 15, wherein the hydrophilic compound is gelatin, alginic acid, chitosan, dextran, polyvinyl alcohol, polyethylene glycol or a derivative thereof, carrageenan, agarose, polyacrylic acid, or polyacrylamide.

20. A method for producing the biosensor according to claim 15, which comprises steps of coating a substrate with a hydrophobic polymer, and allowing a hydrophilic compound to bind to the hydrophobic polymer coated on the substrate.

21. The biosensor according to claim 15, wherein a physiologically active substance is bound to the surface by covalent bonding.

22. A method for immobilizing a physiologically active substance on a biosensor, which comprises a step of allowing a physiologically active substance to come into contact with the biosensor according to claim 15, so as to allow said physiologically active substance to bind to the surface of said biosensor via a covalent bond.

23. A method for detecting or measuring a substance interacting with a physiologically active substance, which comprises a step of allowing a test substance to come into contact with the biosensor according to claim 15 to the surface of which the physiologically active substance binds via a covalent bond.

24. The method according to claim 23, wherein the substance interacting with the physiologically active substance is detected or measured by a non-electrochemical method.

25. The method according to claim 23, wherein the substance interacting with the physiologically active substance is detected or measured by surface plasmon resonance analysis.

26. A biosensor comprising a substrate coated with a hydrophobic polymer, wherein at least two types of surfaces are patterned in a region coated with a hydrophobic polymer by ink-jet printing.

27. The biosensor surface according to claim 26, wherein at least one type of linker is patterned on the surface of the substrate coated with the hydrophobic polymer.

28. The biosensor surface according to claim 26, which is characterized in that the linker is represented by the formula (1):

X-L-Y  

 

Formula (1);

wherein X represents a group capable of reacting with a functional group of the hydrophobic polymer, L represents a bivalent linking group, and Y represents a group capable of immobilizing a physiologically active substance.

29. The biosensor according to claim 26, wherein a physiologically active substance is bound to the surface by covalent bonding.

30. The biosensor according to claim 29, wherein at least a measurement unit to which a physiologically active substance or a substance interacting therewith binds and a measurement unit that does not have a physiologically active substance or a substance interacting therewith exit on a single plane.

31. A method for immobilizing a physiologically active substance on a biosensor, which comprises a step of allowing a physiologically active substance to come into contact with the biosensor according to claim 26, so as to allow said physiologically active substance to bind to the surface of said biosensor via a covalent bond.

32. A method for detecting or measuring a substance interacting with a physiologically active substance, which comprises a step of allowing a test substance to come into contact with the biosensor according to claim 26 to the surface of which the physiologically active substance binds via a covalent bond.

33. The method according to claim 32, wherein the substance interacting with the physiologically active substance is detected or measured by a non-electrochemical method.

34. The method according to claim 32, wherein the substance interacting with the physiologically active substance is detected or measured by surface plasmon resonance analysis.