Method of reducing injury to mammalian cells
First Claim
1. An inhibitor comprising an isolated, recombinant or synthetic polypeptide that inhibits binding between a TRP channel protein and a TRP-associated protein.
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Abstract
This invention relates to methods of reducing the damaging effect of an injury to mammalian cells by treatment with compounds which reduce cell death or dysfunction, including cellular damage following episodes of tissue ischemia, trauma, epilepsy, and acute or chronic degeneration. The invention discloses methods of treating these disorders by administering inhibitors that disrupt protein-protein interactions involved in these disorders, screening methods to identify such inhibitors and specific compositions useful for treating these disorders.
67 Citations
41 Claims
- 1. An inhibitor comprising an isolated, recombinant or synthetic polypeptide that inhibits binding between a TRP channel protein and a TRP-associated protein.
- 13. An inhibitor comprising a nucleic acid sequence capable of inhibiting the expression of a TRP channel protein post transcriptionally.
- 16. The inhibitor of 15, wherein the nucleic acid sequence is coupled to a delivery system selected from the group consisting of an adenovirus vector and an adeno-associated virus vector.
- 19. A method of treating mammalian cell injury, comprising introducing a modulator of binding between a TRP channel protein and a TRP channel associated protein into a cell.
- 26. A method of reducing the damaging effect of ischemia or traumatic injury to the brain or spinal cord in a mammal, said method comprising treating said mammal with a non-toxic, damage-reducing, effective amount of a modulator of binding between a TRP channel protein and a TRP channel associated protein.
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39. A method of controlling the concentration of Ca2+-dependent signaling molecules in the vicinity of ion channel pores of cells in vivo to prevent the diffusion of toxic amounts of said Ca2+ influx to prevent the triggering of neurotoxic phenomena, said method comprising administering an effective, non-toxic amount of a modulator of TRP channel proteins or cellular protein interaction domains that effect said TRP channel protein interactions.
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40. A method for determining whether a test compound modulates binding between a TRP channel protein and a PDZ domain-containing polypeptide, comprising:
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i. contacting a TRP channel PDZ-Ligand sequence with a PDZ domain-containing polypeptide; and
ii. measuring the amount of complex formed between the TRP channel PDZ-Ligand sequence and the PDZ domain-containing polypeptide. - View Dependent Claims (41)
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Specification