Micro-volume blood sampling device

• US 20050178218A1
• Filed: 01/26/2005
• Published: 08/18/2005
• Est. Priority Date: 01/28/2004
• Status: Abandoned Application

• Current Assignee: Avantra Biosciences Corporation
• Original Assignee: Avantra Biosciences Corporation
• Inventors: Montagu, Jean
• Application Number:
  US11/045,425
• Publication Number:
  US 20050178218A1
• Time in Patent Office: Days
• US Class Current: 73/864.340
• CPC Class Codes: A61B 5/150022 for capillary blood or interstitial fluid
• CPC Class Codes: A61B 5/150213 Venting means
• CPC Class Codes: A61B 5/150251 Collection chamber divided into at least two compartments, e.g. for division of samples injection syringes having more than one chamber A61M5/19; syringes for injection of two or more media A61M5/2448, A61M5/284; sample preparation for further analysis A61B5/150755
• CPC Class Codes: A61B 5/150343 Collection vessels for collecting blood samples from the skin surface, e.g. test tubes, cuvettes sampling from within body cavities A61B5/150015, A61B5/150045
• CPC Class Codes: A61B 5/150351 Caps, stoppers or lids for sealing or closing a blood collection vessel or container, e.g. a test-tube or syringe barrel caps for bottles or containers in general B65D41/00
• CPC Class Codes: A61B 5/150755 Blood sample preparation for further analysis, e.g. by separating blood components or by mixing
• CPC Class Codes: A61B 5/150786 Optical identification systems, e.g. bar codes, colour codes
• CPC Class Codes: A61B 5/150969 Low-profile devices which resemble patches or plasters, e.g. also allowing collection of blood samples for testing
• CPC Class Codes: B01L 2200/027 for microfluidic devices
• CPC Class Codes: B01L 2300/021 Identification, e.g. bar codes
• CPC Class Codes: B01L 2400/0406 capillary forces
• CPC Class Codes: B01L 2400/0409 centrifugal forces
• CPC Class Codes: B01L 2400/0481 squeezing of channels or chambers
• CPC Class Codes: B01L 2400/0688 surface tension valves, capillary stop, capillary break
• CPC Class Codes: B01L 3/502715 characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
• CPC Class Codes: B01L 3/502723 characterised by venting arrangements
• CPC Class Codes: B01L 3/50273 characterised by the means or forces applied to move the fluids micropumps F04B19/006, of the membrane type F04B43/043

Abstract

The invention features a single tool for collecting a known volume of a fluid biological sample from an animal, such as a human or small mammal, for mixing the sample with a predetermined amount of a reagent or a diluent, physically separate components of the sample, for storing the sample in a secure and stable fashion, and for then recovering some or all of the sample in a condition suitable for performing a diagnostic assay. The tool can be equipped with an optional means of recording or displaying information about the sample, such as a tag, a bar code, or a surface indicator that identifies, e.g., the date, animal number, and any additional information necessary. A second bar coded label to remove and attaché to the cage shall be provided. The cage/blood-sample can be correlated if needed at a later date.

Claims

1. A sample collection device comprising:

a) a receptacle body portion having a sample port;

b) a sample chamber having an interior surface;

c) a fluid conduit, positioned at least partially in said receptacle body, fluidly connecting the sample port with the sample chamber; and

d) a fluid reservoir in fluid communication with said sample chamber; and

e) a vent port in atmospheric communication with said sample port.

2. The device of claim 1, wherein said device further comprises a vent plug in operable alignment with said vent port.

3. The device of claim 1, wherein said device further comprises means for attaching said receptacle body to a centrifuge.

4. The device of claim 1, wherein said receptacle body portion further comprises a cavity in fluid communication with said sample chamber and centrifugally distal to said conduit.

5. The device of claim 1, further comprising a solute in said fluid reservoir.

6. The device of claim 5, wherein said solute is a dilution fluid.

7. The device of claim 5, wherein said solute further comprises a stabilizing reagent.

8. The device of claim 5, wherein said solute further comprises an analyte detection reagent.

9. The device of claim 1, wherein said device comprises a plurality of fluid reservoirs.

10. The device of claim 1, wherein said device further comprises a dry reagent on at least a portion of said interior surface of said sample chamber.

11. The device of claim 1, wherein said fluid conduit has a volume, and sample collection device has a sample collection capacity approximately equivalent to said fluid conduit volume.

12. The device of claim 1, wherein said sample chamber has an interior surface, at least a portion of said interior surface being hydrophobic.

13. The device of claim 1, wherein said fluid conduit comprises an interior surface characterized as being hydrophilic.

14. A sample collection device comprising a conduit portion and a receptacle body portion in fluid communication with said conduit portion, said conduit portion having a volume of less than 200 microliters, and said receptacle body portion comprising, affixed to said receptacle body portion:

a) means for allowing a fluid sample to enter said receptacle portion via said conduit portion; and

b) means for forcing said fluid sample to exit said receptacle portion via said conduit portion.

15. The sample collection device of claim 14, further comprising a means for attaching said device to a centrifuge.

16. The sample collection device of claim 14, wherein said receptacle body portion further comprises a fluid reservoir, a resident fluid within said fluid reservoir, and means for allowing said resident fluid to mix with said fluid sample.

17. The sample collection device of claim 14, wherein said receptacle body portion further comprises a means for sequestering a separable component from said fluid sample.

18. The sample collection device of claim 14, wherein said receptacle body portion comprises an interior space having an interior pressure, and further comprises a means for maintaining said interior pressure at atmospheric pressure.

19. The sample collection device of claim 18, wherein said receptacle body further comprises means for increasing and decreasing said interior pressure.

20. The sample collection device of claim 18, wherein said receptacle body portion further comprises means for maintaining said interior pressure at atmospheric pressure.

21. The sample collection device of claim 18, wherein said fluid sample has a vapor phase, and wherein said receptacle body further comprises means for preventing said vapor phase from exiting said device.

22. A method for collecting and recovering a liquid sample, said method comprising the steps of:

a) contacting a liquid sample with the sample collection device of claim 14 so as to allow a known volume of said sample to enter said receptacle body portion of said device;

b) subjecting said sample collection device to centrifugal force; and

c) applying pressure to said receptacle body portion of said device, so as to extrude said sample from said device.