Dry reagent particle assay and device having multiple test zones and method therefor

  • US 20050196875A1
  • Filed: 04/19/2004
  • Published: 09/08/2005
  • Est. Priority Date: 08/09/1995
  • Status: Active Grant
First Claim
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1. A device for determining the presence of at least one of a plurality of analytes in a sample, the device comprising:

  • a test zone corresponding to each analyte selected for determining its presence, each test zone receiving and contacting the sample and a labeled indicator reagent corresponding to the selected analyte with a test zone reagent corresponding to the selected analyte, the test zone reagent corresponding to the selected analyte reacting in the presence of the sample and the labeled indicator reagent corresponding to the selected analyte to form a corresponding test zone reaction product and a corresponding test zone detectable response inversely related to the selected analyte level in the sample;

    a reference zone for receiving from each test zone the labeled indicator reagent not reacted with its corresponding test zone reagent and contacting each labeled indicator reagent with a corresponding reference zone reagent, each reference zone reagent reacting in the presence of the corresponding labeled indicator reagent to form a corresponding reference zone reaction product and a corresponding reference zone detectable response related to each selected analyte level in the sample and related to the corresponding test zone detectable response to establish a substantially constant total detectable response for a pre-determined range of each selected analyte; and

    means for combining the detectable responses from each test zone separately with the corresponding detectable result from the reference zone to determine the selected analyte level in the sample.

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