Bifidobacteria preventing diarrhea caused by pathogenic bacteria

• US 20050260170A1
• Filed: 06/30/2005
• Published: 11/24/2005
• Est. Priority Date: 08/05/1999
• Status: Active Grant

• Current Assignee: Nestec SA (Nestle SA)
• Original Assignee: Nestec SA (Nestle SA)
• Inventors: Rochat, Florence, Reniero, Roberto, Neeser, Jean-Richard, Servin, Alain
• Granted Patent: US 7,767,430 B2
• Time in Patent Office: Days
• US Class Current: 424/93.400
• CPC Class Codes: A23C 19/0323 using only lactic acid bacteria, e.g. Pediococcus and Leuconostoc species; Bifidobacteria; Microbial starters in general using moulds A23C19/0682
• CPC Class Codes: A23C 9/1234 characterised by using a Lactobacillus sp. other than Lactobacillus Bulgaricus, including Bificlobacterium sp.
• CPC Class Codes: A23L 29/065 Microorganisms addition of bacteria for nutritional purposes A23L33/135
• CPC Class Codes: A23V 2400/533 Longum
• CPC Class Codes: A61K 35/745 Bifidobacteria
• CPC Class Codes: C12N 1/205 Bacterial isolates
• CPC Class Codes: C12R 2001/01 Bacteria or Actinomycetales ; using bacteria or Actinomycetales
• CPC Class Codes: Y02A 50/30 Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Abstract

The present invention pertains to novel microorganisms of the genus Bifidobacterium, that are useful in preventing diarrhea brought about by pathogenic bacteria. In particular, the present invention relates to the use of said microorganisms for the preparation of an ingestable support and to a composition containing the same.

Claims

1-8. -8. (canceled)

9. A method of preventing infection in a mammal at risk of infection comprising administering to the mammal a composition including an amount of Bifidobacterium effective to prevent colonization of bacteria in an intestine of the mammal wherein the Bifidobacterium is selected from the group consisting of Bifidobacterium longum CNCM I-2169 and Bifidobacterium longum CNCM I-2170.

10. The method according to claim 9 wherein the bacteria is selected from the group consisting of E. coli, Sigella, Klebsiella, Yersinia, Pseudomonas aeruginosa, Listeria, Streptococcus, Staphilococcus, Clostridium difficile, H. pyori, Candida albicans and Salmonella typhimurium.

11. The method according to claim 9 wherein the composition is administered as a food composition including about 10⁵ cfu/g to about 10¹² cfu/g of the Bifidobacterium.

12. The method according to claim 9 wherein the composition is administered as a pharmaceutical composition including about 10⁷ cfu/g to about 10¹² cfu/g of the Bifidobacterium.

13. The method according to claim 9 wherein the composition includes a supernatant of a culture of the Bifidobacterium.

14. A composition capable of preventing colonization of bacteria causing diarrhea comprising a Bifidobacterium selected from the group consisting of Bifidobacterium longum CNCM I-2169 and Bifidobacterium longum CNCM I-2170.

15. The composition according to claim 14 wherein the composition includes a food composition containing about 10⁵ cfu/g to about 10¹² cfu/g of the Bifidobacterium.

16. The composition according to claim 14 wherein the composition includes a pharmaceutical composition containing about 10⁷ cfu/g to about 10¹² cfu/g of the Bifidobacterium.

17. The composition according to claim 14 wherein the Bifidobacterium comprises a supernatant of a culture of Bifidobacterium.

18. The composition according to claim 14, wherein the Bifidobacterium ranges in concentration from about 1×

10⁶ cells/ml to about 5×

10⁶ cells/ml.

19. A method of preparing a composition capable of preventing colonization of bacteria causing diarrhea in a mammal comprising:

providing an ingestible material; and

adding a therapeutically effective amount of a Bifidobacterium selected from the group consisting of Bifidobacterium longum CNCM I-2169 and Bifidobacterium longum CNCM I-2170 to the ingestible material.

20. The method according to claim 19 wherein the amount of Bifidobacterium ranges from about 10⁵ cfu/g to about 10¹² cfu/g.

21. The method according to claim 19 wherein the ingestible material is selected from the group consisting of a food composition and a pharmaceutical composition.

22. A method of preparing a composition containing a Bifidobacterium capable of preventing colonization of bacteria causing diarrhea in a mammal comprising:

providing an ingestible material;

growing a culture of the Bifidobacterium selected from the group consisting of Bifidobacterium longum CNCM I-2169 and Bifidobacterium longum CNCM I-2170;

forming a supernatant of the culture; and

adding a therapeutically effective amount of the supernatant to the ingestible material.

23. The method according to claim 22 wherein the ingestible material is selected from the group consisting of a food composition and a pharmaceutical composition.

24. The method according to claim 22 wherein the amount of Bifidobacterium ranges from about 10⁵ cfu/g to about 10¹² cfu/g.