Chip based diagnostic device

• US 20060002818A1
• Filed: 06/28/2005
• Published: 01/05/2006
• Est. Priority Date: 07/02/2004
• Status: Active Grant

• Current Assignee: Roche Molecular Systems (Roche Holding AG)
• Original Assignee: Roche Molecular Systems (Roche Holding AG)
• Inventors: Belz, Renato, Carsten, Haack
• Granted Patent: US 7,820,103 B2
• Time in Patent Office: Days
• US Class Current: 422/57
• CPC Class Codes: B01J 2219/00495 Means for heating or cooling the reaction vessels
• CPC Class Codes: B01J 2219/00605 the compounds being directly bound or immobilised to solid supports
• CPC Class Codes: B01J 2219/00637 by coating it with another layer
• CPC Class Codes: B01J 2219/00722 Nucleotides
• CPC Class Codes: B01L 2200/025 Align devices or objects to ensure defined positions relative to each other
• CPC Class Codes: B01L 2200/0689 Sealing
• CPC Class Codes: B01L 2300/0809 rectangular shaped
• CPC Class Codes: B01L 2300/0819 Microarrays; Biochips
• CPC Class Codes: B01L 3/502 with fluid transport, e.g. in multi-compartment structures centrifugal-type cuvettes G01N21/07; analysis by separation into components G01N30/00; automatic analysers G01N35/00
• CPC Class Codes: B01L 9/52 Supports specially adapted for flat sample carriers, e.g. for plates, slides, chips
• CPC Class Codes: G01N 2035/00158 Elements containing microarrays, i.e. "biochip"
• CPC Class Codes: Y10T 436/25375 Liberation or purification of sample or separation of material from a sample [e.g., filtering, centrifuging, etc.]

Abstract

A diagnostic device comprising a sample inlet and a processing chamber having a window portion and a flat carrier having front surface, a back surface and one or more side surfaces, said front surface facing said processing chamber, said front surface having an active surface containing a diagnostic reagent immobilized to said active front surface and an extended front surface, wherein said window portion further contains a rim portion facing said extended front surface sealing said chamber versus said carrier through said extended front surface.

Claims

1. A diagnostic device comprising a sample inlet and a processing chamber having a window portion and a flat carrier having front surface facing said processing chamber, said front surface having an active surface containing a diagnostic reagent immobilized to said active front surface and an extended front surface, wherein said window portion further contains a rim portion facing said extended front surface sealing said chamber versus said carrier through said extended front surface, further comprising a locking frame to push the carrier towards said rim portion up to a dead stop position.

2. The device according to claim 1, wherein said rim portion contains a circumferential wall made of an elastic material.

3. The device according to claim 1, wherein said locking frame is flat containing metal parts.

4. The device according to claim 1, wherein the sample inlet is closed by a cap comprising a pierceable membrane.

5. The device according to claim 1, wherein the processing chamber is a mixing chamber.

8. A method for determining the presence of an analyte in a sample in an instrument comprising the steps:

providing a device according to claim 1, positioning said device within said instrument,

placing said sample into said device such that said sample is in contact with the active surface of said carrier,

reacting said analyte with said diagnostic reagent,

determining the results of said reaction as a measure of the presence of said analyte in said sample.

9. The method of claim 8, further comprising the step of removing liquid from said processing chamber.

10. The method of claim 8, further comprising a step of dispensing and aspirating a washing liquid to said processing chamber.

11. The method of claim 8, further comprising mixing any liquid in said processing chamber.

12. The method of claim 11, wherein said mixing is done by vortex shaking.

13. A diagnostic instrument for determining the presence of an analyte in a sample comprising:

one or more devices according to claim 1,

a module for entering a sample into said device, and

a module for determining the results of the reaction of analytes in said sample with said reagents.

6. A method for assembling a diagnostic device comprising a processing chamber having a window portion and a flat carrier having front surface, a back surface and one or more side surfaces, said front surface having an active surface containing a diagnostic reagent immobilized to said active front surface and an extended front surface, comprising:

providing a device comprising a processing chamber having an open window portion, said window portion further containing a rim portion,

positioning said device,

providing said flat carrier,

placing the front surface of said carrier on to said rim portion such that said front surface faces said processing chamber and said rim seals said chamber versus said carrier through said extended front surface,

providing a locking frame having clips spanning corners of said frame, and

pressing said locking frame towards the device such that it is retained in a position that fixes the carrier.

7. The method of claim 6 further comprising labeling the device.

14. An article of manufacture made from a single material for locking a flat carrier to an analytical device comprising:

a substantially rectangular frame,

two or more clips spanning the corners of the rectangle being more flexible then the frame.

15. The article of claim 14 wherein the clip is a brace bridging a corner by connecting two adjacent sides of the rectangle.

16. The article of claim 14, wherein there is a gap between each clip and the corner it spans.

17. The article of claim 14, further comprising positioning means.

18. The article of claim 14, further comprising a window formed by said frame and the clips which is larger then 99% of the surface of the flat carrier.