Frequency-multiplexed detection of multiple wavelength light for flow cytometry
First Claim
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1. A multiple wavelength light system comprising:
- a plurality of light sources;
an at least one mirror proximate to the plurality of light sources;
at least one multiplexer connected to the plurality of light sources;
a detector proximate to a target; and
a demultiplexer connected to the detector.
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Abstract
A multiplexed set of light sources having outputs of light with various wavelengths which are combined into one beam. The beam may impinge a particle in a flow channel of a cytometer. The light leaving the flow channel may be sensed by a detector and the light distinguished according to wavelength.
91 Citations
37 Claims
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1. A multiple wavelength light system comprising:
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a plurality of light sources;
an at least one mirror proximate to the plurality of light sources;
at least one multiplexer connected to the plurality of light sources;
a detector proximate to a target; and
a demultiplexer connected to the detector. - View Dependent Claims (2, 3, 4, 5, 6)
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7. A multiple wavelength light system comprising:
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a first light source having a first wavelength;
a second light source having a second wavelength;
a first modulator, having a first modulation frequency, connected to the first light source;
a second modulator, having a second modulation frequency, connected to the second light source;
a light combiner proximate to the first and second light sources;
a detector mechanism proximate to an output of the light combiner; and
a frequency demodulator connected to the detector. - View Dependent Claims (8, 9, 10, 11, 12)
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13. A multiple wavelength optical system comprising:
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a plurality of light sources;
a frequency modulator connected to each light source;
a dichroic fold mirror having an element proximate to each light source, and an output of light directed to a target;
a light detector proximate to the target; and
a frequency analyzer connected to the light detector. - View Dependent Claims (14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24)
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25. A method for identifying components of a detected light beam having different wavelengths, comprising:
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modulating with a first frequency a first light having a first wavelength;
modulating with another frequency at least another light having another wavelength;
combining the light having the first wavelength with the at least another light with the another wavelength into a light beam;
detecting the light beam with a detector that converts the detected light into an electrical signal; and
analyzing the electrical signal into signals representing light of the first wavelength and signals representing the at least another light having another wavelength. - View Dependent Claims (26, 27, 28, 29, 30, 31)
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32. Means for identifying components of a light according to wavelength from an electrical signal representing the light, comprising:
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means for providing a first light having a first wavelength;
means for providing at least another light having another wavelength;
means for modulating the first light with a first frequency;
means for modulating the at least another light with another frequency;
means for combining the first light and the at least another light into a single light;
means for converting the single light into an electrical signal; and
means for analyzing the electrical signal into a first signal that represents the first light and at least another signal that represents the at least another light, according to the first frequency and another frequency, respectively. - View Dependent Claims (33, 34, 35, 36, 37)
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Specification