Mobilization of cells via physical means

• US 20060051328A1
• Filed: 08/23/2005
• Published: 03/09/2006
• Est. Priority Date: 09/07/2004
• Status: Abandoned Application

• Current Assignee: Lanny L. Johnson
• Original Assignee: Lanny L. Johnson
• Inventors: Johnson, Lanny L.
• Application Number:
  US11/210,078
• Publication Number:
  US 20060051328A1
• Time in Patent Office: Days
• US Class Current: 424/93.700
• CPC Class Codes: A61K 35/15 Cells of the myeloid line, e.g. granulocytes, basophils, eosinophils, neutrophils, leucocytes, monocytes, macrophages or mast cells; Myeloid precursor cells; Antigen-presenting cells, e.g. dendritic cells presenting a specific antigen A61K39/00; therapeutic combinations of antibodies, or fragments thereof, and blood-derived cells A61K39/00
• CPC Class Codes: A61K 35/28 Bone marrow; Haematopoietic stem cells; Mesenchymal stem cells of any origin, e.g. adipose-derived stem cells
• CPC Class Codes: A61K 39/461 characterised by the cell type used
• CPC Class Codes: A61K 39/464 characterised by the antigen targeted or presented
• CPC Class Codes: A61K 41/0028 Disruption, e.g. by heat or ultrasounds, sonophysical or sonochemical activation, e.g. thermosensitive or heat-sensitive liposomes, disruption of calculi with a medicinal preparation and ultrasounds

Abstract

A method for treating a patient to repair or regenerate damaged tissue which comprises exposing the patient to an internal physical treatment of the body, for example ultrasound waves or ultra shock waves, MRI or bone surgery so that stem cells, progenitor cells and/or macrophages are released into the bloodstream of the patient due to the internal physical treatment, harvesting the cells from the bloodstream of the patient, and introducing the cells into the damaged tissue of the patient.

Claims

1. A method for treating a patient to repair damaged tissue which comprises:

(a) exposing a selected area of bone of a patient to ultrasound waves or ultra shock waves so that cells comprising stem cells, progenitor cells or macrophages are released into the bloodstream of the patient from the area due to the ultrasound;

(b) harvesting the cells from the bloodstream of the patient; and

(c) introducing the cells to the damaged tissue of the patient so as to repair the damaged tissue.

2. The method of claim 1 wherein the area comprises the bone in a trunk or extremity of the patient so that the cells are released from marrow of the bone.

3. The method of claim 1 wherein the cells are introduced to an organ as the damaged tissue.

4. The method of claim 1 wherein the cells are introduced to cartilage as the damaged tissue.

5. The method of claim 1 wherein the cells are introduced to bone as the damaged tissue.

6. The method of claim 1 wherein the cells are introduced to bone marrow as the damaged tissue.

7. The method of any one of claims 1-6 wherein the patient is a human.

8. The method of any one of claims 1-6 wherein the patient is an animal.

9. The method of claim 1 wherein the shock waves are from a lithotripsy apparatus which are directed into the area.

10. The method of claim 9 wherein the area is the bone in an extremity of the patient.

11. The method of claim 10 wherein the bone is in an arm or a leg.

12. The method of claim 9 wherein the area is the bone in a trunk of the patient.

13. The method of claim 12 wherein the bone is a sternum or an iliac crest.

14. A method for treating a patient to repair damaged tissue which comprises:

(a) exposing a kidney stone in a patient to ultrasound waves or ultra shock waves so that cells comprising stem cells, progenitor cells or macrophages are released into the bloodstream of the patient from the area due to the ultrasound;

(b) harvesting the cells from the bloodstream of the patient; and

(c) introducing the cells into the damaged tissue of the patient so as to repair the damaged tissue.

15. A method for treating a recipient patient to repair damaged tissue which comprises:

(a) exposing an area in a donor patient to ultrasound waves or ultra shock waves so that cells comprising stem cells, progenitor cells or macrophages are released into the bloodstream of the donor from the area due to the ultrasound;

(b) harvesting the cells from the bloodstream of the donor patient; and

(c) introducing the cells into the damaged tissue of the recipient patient so as to repair the damaged tissue.

16. A system for harvesting stem cells, pluripotential cells, progenitor cells or macrophages which comprises:

(a) a container for a bath which provides ultrasound waves or shock waves to an area of an extremity of a patient immersed in the bath so as to generate cells comprising stem cells, pluripotent cells, progenitor cells, macrophages and mixtures thereof in the bloodstream;

(b) harvesting means for removing the cells from the bloodstream.

17. The system of claim 16 further comprising a fluid for submersing the extremity of the patient.

18. The system of claim 16 wherein the bath is for an arm or a leg.

19. A method for treating a patient to repair damaged tissue which comprises:

(a) providing a selected area of the patient to be exposed; and

(b) exposing the selected area of the patient to a physical treatment of the body selected from the group consisting of ultrasound waves, ultra shock waves, bone surgery, CAT scan and magnetic resonance imaging (MRI) so that cells comprising stem cells, progenitor cells or macrophages are released into the bloodstream of the patient from the area due to the physical treatment and such that the stem cells, progenitor cells or macrophages migrate to the damaged tissue of the patient so as to repair the damaged tissue.

20. The method of claim 19 wherein the damaged tissue is a muscle.

21. The method of claim 19 wherein the damaged tissue is a ligament.

22. The method of claim 19 wherein the damaged tissue is a tendon.

23. The method of any one of claims 20, 21 or 22 wherein the cell is a fibroblast.

24. A method for providing a store of stem cells, progenitor cells or macrophages of a patient for future use which comprises:

(a) exposing a selected area of a patient to a physical treatment of the body selected from the group consisting of ultrasound waves, ultra shock waves, bone surgery, CAT scan and magnetic resonance imaging (MRI) so that cells comprising stem cells, progenitor cells or macrophages are released into the bloodstream of the patient from the area due to the ultrasound;

(b) harvesting the cells from the bloodstream of the patient; and

(c) freezing the cells harvested from the bloodstream of the patient so as to provide a store of stem cells, progenitor cells or macrophages of the patient for future use.

25. A method for treating a patient to repair damaged tissue which comprises:

(a) performing a surgical procedure upon a selected area of bone of a patient so that cells are released from bone marrow into the bloodstream of the patient from the area due to the surgical procedure;

(b) harvesting the cells from the patient; and

(c) introducing the cells to the damaged tissue of the patient so as to repair the damaged tissue.

26. The method of claim 25 wherein the surgical procedure is total joint surgery.

27. The method of claim 25 wherein the surgical procedure is open reduction internal fixation (ORIF).

28. The method of claim 25 wherein the cells are harvested directly from marrow exposed during the surgical procedure.

29. The method of claim 28 wherein the cells are stem cells, progenitor cells, macrophages or precursors of macrophages.

30. The method of claim 25 wherein the cells are harvested from the bloodstream of the patient.

31. A method for treating a patient to repair damaged tissue which comprises:

(a) performing a surgical procedure upon a selected area of bone of a patient;

(b) harvesting bone marrow cells from the patient;

(c) isolating a population of cells from the bone marrow cells; and

(c) introducing isolated population of cells to the damaged tissue of the patient so as to repair the damaged tissue.

32. The method of claim 31 wherein the surgical procedure is total joint surgery.

33. The method of claim 31 wherein the surgical procedure is open reduction internal fixation (ORIF).

34. The method of claim 31 wherein the isolated population of cells are stem cells, progenitor cells, macrophages or precursors of macrophages.

35. A method for treating a patient to repair damaged tissue which comprises:

(a) exposing a patient to an external magnetic field and an applied oscillating electromagnetic field so that cells comprising stem cells, progenitor cells or macrophages are released into the bloodstream of the patient due to the exposure;

(b) harvesting the cells from the patient; and

(c) introducing the cells to the damaged tissue of the patient so as to repair the damaged tissue.

36. The method of claim 35 wherein the patient is exposed to the external magnetic field and the applied oscillating electromagnetic field during a magnetic resonance imaging (MRI) procedure.

37. A surgical instrument for collecting bone marrow tissue having a proximal end and an opposing distal end, the instrument comprising:

(a.) a handle at the proximal end of the surgical instrument for gripping by a surgeon;

(b.) an elongate hollow tube attached to the handle, the hollow tube having an opening at a first end of the hollow tube for attachment to a vacuum means, and a pointed tip at a second end of the hollow tube, the pointed tip situated at the distal end of the surgical instrument;

(c.) one or more distal openings in the hollow tube adjacent to the tip which allow fat and cells to enter the tube when a suction is provided by the vacuum means connected to the open end of the hollow tube so as to draw out the marrow tissue from the bone for collection; and

(d.) one or more secondary slits along the hollow tube which provide venting so as to avoid clogging of the one or more distal openings when the suction is provided by the vacuum means.

38. The instrument of claim 37 wherein the handle comprises:

(a) a grip having a first end and a second end for gripping by the surgeon; and

(b) a shaft attached to the hollow tube, wherein the grip is attached perpendicularly to the shaft equidistant between the first end and the second end so as to form a T-shape.