Dendritic cell nodes

• US 20060078540A1
• Filed: 03/18/2003
• Published: 04/13/2006
• Est. Priority Date: 03/18/2002
• Status: Abandoned Application

• Current Assignee: Dana-Farber Cancer Institute, Massachusetts Institute of Technology, Sanofi Pasteur VaxDesign Corp. (Sanofi ), Whitehead Institute for Biomedical Research
• Original Assignee: Sanofi Pasteur VaxDesign Corp. (Sanofi )
• Inventors: Huang, Qian, Kachurin, Anatoly, Hacohen, Nir, Warren, William L, Chen, Lan Bo, Irvine, Darell, Higbee, Russell G
• Application Number:
  US10/508,350
• Publication Number:
  US 20060078540A1
• Time in Patent Office: Days
• US Class Current: 424/93.100
• CPC Class Codes: A61K 2039/5154 Antigen presenting cells [APCs], e.g. dendritic cells or macrophages
• CPC Class Codes: C12N 2502/11 blood or immune system cells
• CPC Class Codes: C12N 5/0639 Dendritic cells, e.g. Langherhans cells in the epidermis
• CPC Class Codes: C12N 5/0697 Artificial constructs associating cells of different lineages, e.g. tissue equivalents blood vessels C12N5/0691

Abstract

The present invention features dendritic cell nodes that can be used to vaccinate subjects against pathogens and to modulate a subject'"'"'s immune system to treat or prevent various diseases and conditions.

Claims

1. A dendritic cell node comprising:

a) a biocompatible scaffold material;

b) a chemokine for attracting immature dendritic cells;

c) a chosen antigen; and

d) a maturation signal for dendritic cells.

2-25. -25. (canceled)

26. A method of constructing a dendritic cell node comprising:

a) depositing, onto a substrate, a layer for attracting monocytes into the dendritic cell node;

b) depositing, onto layer (a), a layer for inducing differentiation of the monocytes into immature dendritic cells;

c) depositing, onto layer (b), a layer for presenting a chosen antigen to immature dendritic cells;

d) depositing, onto layer (c), a layer for attracting dendritic cells and inducing maturation of dendritic cells, thereby constructing a dendritic cell node.

27. (canceled)

28. A method of constructing a dendritic cell node comprising:

a) depositing, onto a substrate, a layer for attracting immature dendritic cells into the dendritic cell node;

b) depositing, onto layer (a), a layer for presenting a chosen antigen to the immature dendritic cells; and

c) depositing, onto layer (b), a layer for attracting immature dendritic cells and inducing maturation of the immature dendritic cells;

or

d) depositing, onto a substrate, a layer for attracting immature dendritic cells and inducing maturation of the immature dendritic cells;

e) depositing, onto layer (d), a layer for presenting a chosen antigen to the immature dendritic cells; and

f) depositing, onto layer (e), a layer for attracting immature dendritic cells into the dendritic cell node, thereby constructing an dendritic cell node.

29-47. -47. (canceled)