Methods and products related to the intracellular delivery of polysaccharides
First Claim
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1. A composition, comprising:
- a cationic polymer, and a polysaccharide, wherein the polysaccharide is present in a intracellular therapeutically effective amount.
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Abstract
The invention relates, in part, to methods and compositions for the intracellular delivery of polysaccharides. In particular, the methods and compositions relate to the intracellular delivery of glycosaminoglycans, such as heparin. The invention in other aspects relates to the use of glycosaminoglycans for the treatment of proliferative disorders, such as cancer. The invention is still other aspects relates to improving cell viability. The invention also relates to the delivery of polysaccharides while avoiding unwanted effects of the polysaccharides. For example, heparin can be delivered while avoiding its anticoagulant effects.
118 Citations
108 Claims
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1. A composition, comprising:
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a cationic polymer, and a polysaccharide, wherein the polysaccharide is present in a intracellular therapeutically effective amount. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10)
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11-31. -31. (canceled)
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32. A composition, comprising:
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a polysaccharide, a cationic polymer, and a pharmaceutically acceptable carrier, wherein more cationic polymer is present in the composition (w/w) than the polysaccharide.
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33-41. -41. (canceled)
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42. A composition, comprising:
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a poly(β
-amino ester), anda polysaccharide. - View Dependent Claims (101)
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43-50. -50. (canceled)
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51. A composition, comprising:
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a cationic polymer, a polysaccharide, and a targeting molecule.
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52-59. -59. (canceled)
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60. A method for the intracellular delivery of a therapeutically effective amount of a polysaccharide, comprising:
administering the polysaccharide complexed to a cationic polymer to promote the uptake of the polysaccharide into a cell in a therapeutically effective amount.
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61-77. -77. (canceled)
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78. A method for promoting apoptosis in a subject, comprising:
administering a glycosaminoglycan intracellularly in an amount effective to promote apoptosis, wherein the glycosaminoglycan is delivered in an intracellular therapeutically effective amount to promote apoptosis.
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79-87. -87. (canceled)
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88. A method for treating a disease characterized by abnormal cell proliferation in a subject, comprising:
administering a glycosaminoglycan intracellularly in an amount effective to treat the disease, wherein the glycosaminoglycan is delivered in an intracellular therapeutically effective amount to treat the disease.
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89-100. -100. (canceled)
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102. (canceled)
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103. A method for the intracellular delivery of a polysaccharide, comprising:
administering the polysaccharide complexed to a cationic polymer to a non-macrophage cell, wherein the polysaccharide is not present in excess of the cationic polymer.
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104. A method of promoting cell viability, comprising:
contacting a cell with a cationic polymer-polysaccharide conjugate prior to freezing the cell in an amount effective to increase the cell'"'"'s viability when thawed.
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105-106. -106. (canceled)
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107. A method for inhibiting abnormal cell proliferation, comprising:
administering a glycosaminoglycan complexed to a cationic polymer in an amount effective to inhibit cell proliferation.
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108-120. -120. (canceled)
Specification