Diagnostic test using gated measurement of fluorescence from quantum dots

• US 20060128034A1
• Filed: 12/10/2004
• Published: 06/15/2006
• Est. Priority Date: 12/10/2004
• Status: Abandoned Application

• Current Assignee: Alverix, Inc. (Becton, Dickinson & Co)
• Original Assignee: Alverix, Inc. (Becton, Dickinson & Co)
• Inventors: Bruchez, Marcel P., Zhou, Rong, Roitman, Daniel B., Petruno, Patrick T., Petrilla, John F., Watson, Andrew R.
• Application Number:
  US11/008,912
• Publication Number:
  US 20060128034A1
• Time in Patent Office: Days
• US Class Current: 436/524
• CPC Class Codes: G01N 21/6428 Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" in vivo A61B5/00; immunoassay G01N33/53
• CPC Class Codes: G01N 21/8483 Investigating reagent band test-element handling not specific to a test method G01N33/4875; analytical elements specific to chemical analysis of biological material G01N33/52; autometer with reagent band G01N35/04

Abstract

A rapid diagnostic test system or process uses a gated measurement of the fluorescent light from quantum dots after shutting off an illuminating light source. A delay between shutting off the illumination and measuring allows background fluorescence from substances other than the quantum dots to drop significantly when compared to the intensity of the fluorescence from the quantum dots. Using quantum dots permits high measurement repetition rates and good extinction of background fluorescence.

Claims

1. A rapid diagnostic test system comprising:

a light source for illuminating a medium containing a sample under test, wherein the medium comprises a labeling substance that binds a quantum dot to a target analyte;

a first photodetector positioned to measure light from a test area of the medium; and

a control system coupled to the light source and the photodetector, wherein the control system executes a measurement processes including processing a measurement signal from the photodetector that indicates a light intensity after the light source has been off for a time.

2. The system of claim 1, wherein the light that the first photodetector measures has a frequency characteristic of fluorescent light from the quantum dot.

3. The system of claim 2, wherein the medium comprises a lateral-flow strip for performing a binding assay, and the test area contains an immobilized substance that binds to and holds a complex including the labeling substance and the target analyte.

4. The system of claim 1, wherein the control system processes multiple measurements from the photodetector that indicate the light intensity after the light source has been off for the time.

5. The system of claim 1, further comprising:

a second photodetector; and

an optical system positioned to receive light from the test area, wherein the optical system separates light having a first frequency from light having a second frequency so that the first photodetector measures light having the first frequency and the second photodetector measures light having the second frequency.

6. The system of claim 5, wherein the optical system comprises a diffractive element that directs the light of the first frequency on the first photodetector and directs the light of the second frequency on the second photodetector.

7. The system of claim 5, wherein the optical system comprises a color filter that transmits light having one of the first and second frequencies and reflects light having the other of the first and second frequencies.

8. The system of claim 6, wherein the quantum dot emits fluorescent light having the first frequency;

and wherein the medium further comprises a second labeling substance containing a second quantum dot emits fluorescent light having the second frequency.

9. The system of claim 1, wherein the first photodetector comprises a portion of an imaging array that captures an image containing the test area of the medium.

10. The system of claim 1, wherein the first photodetector and the medium are contained in a single-use module.

15. The process of claim 11, further comprising activating a display on the single-use module to indicate the test result signal.

11. A process for rapid diagnostic testing, comprising:

applying a sample to a medium containing a labeling substance that binds a quantum dot to a target analyte;

illuminating a portion of the medium with light capable of causing the quantum dot to fluoresce;

stopping illumination of the portion of the medium;

measuring light from the portion of the medium after the illumination remains stopped for a delay time; and

determining a test result from the measuring of the light.

12. The process of claim 11, further comprising repeating the illuminating, stopping illumination, and measuring steps a plurality of times, wherein determining the test result uses results from each repetition of measuring the light.

13. The process of claim 12, wherein the measuring step is repeated at a frequency between about 1 MHz and about 200 MHz.

14. The process of claim 11, wherein the medium is in a single-use structure that includes a photodetector that measures light from the quantum dot.

16. The process of claim 11, further comprising producing an electrical signal that is output from the single-use structure.