Expansion of definitive endoderm cells
First Claim
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1. A method of expanding definitive endoderm cells in culture, said method comprising the steps of;
- (a) obtaining a cell culture comprising definitive endoderm cells;
(b) isolating at least some of the definitive endoderm cells from at least some of the other cells in the cell culture, thereby producing a cell population enriched in definitive endoderm cells; and
(c) culturing said cell population enriched in definitive endoderm cells under conditions that permit the expansion of said definitive endoderm cells.
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Abstract
Disclosed herein are cell cultures comprising expanded definitive endoderm cells as well as methods for expanding definitive endoderm cells in culture.
159 Citations
49 Claims
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1. A method of expanding definitive endoderm cells in culture, said method comprising the steps of;
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(a) obtaining a cell culture comprising definitive endoderm cells;
(b) isolating at least some of the definitive endoderm cells from at least some of the other cells in the cell culture, thereby producing a cell population enriched in definitive endoderm cells; and
(c) culturing said cell population enriched in definitive endoderm cells under conditions that permit the expansion of said definitive endoderm cells. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38)
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39. A method of expanding definitive endoderm cells in culture, said method comprising the steps of:
- (a) obtaining a cell culture comprising definitive endoderm cells, and (b) passaging said definitive endoderm cells, thereby producing a plurality of cell cultures comprising definitive endoderm cells.
- View Dependent Claims (40, 41, 42, 43, 44, 45, 46, 47, 48, 49)
Specification