Biosensor, biosensor system and method of using thereof

• US 20060205060A1
• Filed: 11/23/2005
• Published: 09/14/2006
• Est. Priority Date: 01/11/2005
• Status: Active Grant

• Current Assignee: Samsung Electronics Co. Ltd.
• Original Assignee: Samsung Electronics Co. Ltd.
• Inventors: Kang, Jung-ho, Park, Tae-sik, Kim, Young-il
• Granted Patent: US 7,815,860 B2
• Time in Patent Office: Days
• US Class Current: 435/287.200
• CPC Class Codes: G01N 27/3271 Amperometric enzyme electrodes for analytes in body fluids, e.g. glucose in blood amperometry per se G01N27/49; aspects concerning the enzyme reagent C12Q1/001

Abstract

A biosensor for detecting an analyte by using a variable voltage according to infrared radiation absorption is provided. The biosensor includes an infrared absorber having a variable resistance and bioreceptors immobilized onto the infrared absorber for selectively reacting and binding with an analyte, wherein an induced voltage at the infrared absorber varies depending on whether the bioreceptor reacts and binds with the analyte.

Claims

1. A biosensor, comprising:

an infrared absorber having a variable resistance; and

bioreceptors immobilized onto the infrared absorber for selectively reacting and binding with an analyte,

wherein, an induced voltage at the infrared absorber varies depending on whether the bioreceptor reacts and binds with the analyte.

2. The biosensor according to claim 1, further comprising:

a voltage amplifying part connected to the infrared absorber and configured for amplifying the induced voltage at the infrared absorber.

3. The biosensor according to claim 2, wherein the voltage amplifying part comprises a MOSFET, Bipolar transistor, or a combination including at least one of the foregoing.

4. The biosensor according to claim 2, wherein the infrared absorber and the voltage amplifying part are layered on a wafer.

5. The biosensor according to claim 2, further comprising

an insulating layer formed on the voltage amplifying part;

and a through-hole formed in the insulating layer for connecting the voltage amplifying part to the infrared absorber, the infrared absorber being formed on the insulating layer.

6. The biosensor according to claim 1, wherein the infrared absorber comprises Ti (titanium).

7. The biosensor according to claim 1, wherein the bioreceptor comprises enzyme, antibody, antigen, membrane, receptor, cell, tissue, DNA, or a combination including at least one of the foregoing.

8. A biosensor, comprising:

a substrate;

a plurality of infrared absorbers formed on the substrate,

a plurality of bioreceptors immobilized onto the infrared absorbers, the plurality of bioreceptors being of varying types configured for selectively reacting and binding with different analytes; and

a plurality of voltage amplifying parts connected to the plurality of the infrared absorbers, for amplifying an induced voltage at each infrared absorber,

wherein, the induced voltage at the infrared absorber varies depending on whether the different kinds of bioreceptors react and bind with the analytes.

9. The biosensor according to claim 8, wherein the voltage amplifying part comprises a MOSFET, Bipolar transistor or a combination including at least one of the foregoing.

10. The biosensor according to claim 8, wherein the biosensor is layered on a wafer.

11. The biosensor according to claim 8, wherein the infrared absorber comprises Ti (titanium).

12. The biosensor according to claim 8, wherein the infrared absorber is shaped for maximum infrared radiation absorption,

13. The biosensor according to claim 8, wherein the bioreceptor comprises enzyme, antibody, antigen, membrane, receptor, cell, tissue, DNA, or a combination including at least one of the foregoing.

14. The biosensor according to claim 8, further comprising a plurality of sensor units comprising the plurality of infrared absorbers and the plurality of voltage amplifying parts, the sensor units arranged in a grid pattern.

15. The biosensor according to claim 8, wherein the plurality of infrared absorbers each have a resistance varying in accordance with an amount of absorbed infrared radiation.

16. A biosensor system, comprising:

an infrared generator;

a biosensor including;

an infrared absorber having a variable resistance according to the amount of absorbing infrared radiation emitted from the infrared generator; and

bioreceptors immobilized onto the infrared absorber for selectively reacting and binding with an analyte, wherein, an induced voltage at the infrared absorber varies depending on whether the bioreceptor reacts and binds with the analyte; and

an output unit for displaying an induced voltage at the biosensor.

17. The biosensor system according to claim 16, wherein the biosensor further comprises:

a voltage amplifying part connected to the infrared absorber, for amplifying the induced voltage at the infrared absorber.

18. The biosensor system according to claim 17, wherein the voltage amplifying part comprises a MOSFET, Bipolar transistor, or a combination including at least one of the foregoing.

19. The biosensor system according to claim 18, wherein the infrared absorber and the voltage amplifying part are layered on a wafer.

20. The biosensor system according to claim 16, wherein the infrared generator comprises an infrared lamp.

21. The biosensor system according to claim 16, wherein the infrared absorber comprises Ti (titanium).

22. The biosensor system according to claim 16, wherein the bioreceptor comprises enzyme, antibody, antigen, membrane, receptor, cell, tissue, DNA, or any combination including at least one of the foregoing.

23. The biosensor system according to claim 16, wherein the biosensor further comprises a support plate, the infrared absorbers formed on the support plate.

25. The method according to claim 18, further comprising

prior to the coating a sample on a biosensor, determining whether a bioreceptor is immobilized on an infrared absorber, the biosensor comprising the bioreceptor and the infrared absorber;

irradiating the infrared absorber in a first irradiation and measuring a first voltage induced at the bioreceptor;

disposing the bioreceptor onto the infrared absorber,

irradiating a surface of the biosensor in a second irradiation and measuring a second voltage induced at the biosensor; and

comparing the first voltage to the second voltage to determine whether the bioreceptor is immobilized onto the biosensor;

wherein the bioreceptor is determined immobilized when the first voltage is different than the second voltage; and

wherein the second voltage is the reference voltage.

24. A method of detecting a target object comprising:

coating a sample on a biosensor, the sample including the target object to be detected;

irradiating a surface of the biosensor;

measuring an output voltage induced at the biosensor; and

comparing the output voltage with a reference voltage to determine whether the target object is contained in the sample;

wherein the target object is detected in the sample when the output voltage is different than the reference voltage.