Biosensors having improved sample application and uses thereof
First Claim
Patent Images
1. A biosensor for electrochemical analysis of a liquid sample, which biosensor comprises:
- a) a base member having a proximal end and a distal end;
b) an electrode system comprising a working electrode and a counter electrode, said working and counter electrodes having conductive leads at said proximal end of said base member for connecting said electrodes to a readout device for electrochemical measurement, wherein said working electrode is positioned adjacent to said counter electrode, and there is a gap space between said working and counter electrodes, c) a reaction area which encompasses at least a portion of said working electrode, said counter electrode and said gap space between the working electrode and counter electrode;
d) a dielectric coating positioned between said electrode system and said base member and within at least a portion of the reaction area; and
e) a test reagent in the reaction area, which test reagent is positioned over the dielectric layer, wherein said test reagent comprises a liquid soluble hydrophilic component, and wherein said test reagent further comprises at least one member selected from the group consisting of an electron transfer mediator, an enzyme effective to oxidize an analyte to be analyzed, and a substrate involved in a reaction catalyzed by an enzyme to be analyzed.
0 Assignments
0 Petitions
Accused Products
Abstract
The present invention relates to biosensors having improved sample application and measuring properties and their uses for detection, preferably, quantitative measurement, of analyte in a liquid sample. In particular, the invention provides for a biosensor having a sample application, reaction area and liquid soluble hydrophilic material facilitating the speed and uniformity of sample application, especially small volume sample application, via capillary flow. Methods for assaying analytes or enzymes using the biosensors are further provided.
27 Citations
20 Claims
-
1. A biosensor for electrochemical analysis of a liquid sample, which biosensor comprises:
-
a) a base member having a proximal end and a distal end;
b) an electrode system comprising a working electrode and a counter electrode, said working and counter electrodes having conductive leads at said proximal end of said base member for connecting said electrodes to a readout device for electrochemical measurement, wherein said working electrode is positioned adjacent to said counter electrode, and there is a gap space between said working and counter electrodes, c) a reaction area which encompasses at least a portion of said working electrode, said counter electrode and said gap space between the working electrode and counter electrode;
d) a dielectric coating positioned between said electrode system and said base member and within at least a portion of the reaction area; and
e) a test reagent in the reaction area, which test reagent is positioned over the dielectric layer, wherein said test reagent comprises a liquid soluble hydrophilic component, and wherein said test reagent further comprises at least one member selected from the group consisting of an electron transfer mediator, an enzyme effective to oxidize an analyte to be analyzed, and a substrate involved in a reaction catalyzed by an enzyme to be analyzed. - View Dependent Claims (2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20)
-
Specification